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Sample GSM8119707 Query DataSets for GSM8119707
Status Public on Apr 12, 2024
Title liver, ACSS1K635Q, control diet, biol rep 2
Sample type SRA
 
Source name liver
Organism Mus musculus
Characteristics tissue: liver
Sex: male
strain: C57Bl/6J
genotype: ACSS1K635Q
treatment: control diet
Treatment protocol Mice in the fasted group were food denied for 48 hours, but all mice were given free access to water.
Extracted molecule total RNA
Extraction protocol Qiagen RNEasy Kit
Total RNA samples were used for sequencing library preparation with KAPA RNA HyperPrep kit with RiboErase (HMR) following KAPA RNA library preparation protocol. Each library was bar-coded and then pooled for sequencing run paired-end sequencing protocol.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing Illumina bcl2fastq software used for base-calling
All RNA-seq FastQ reads were aligned with the reference genome (UCSC human genome build hg19) using STAR-RSEM default settings to obtain the counts per gene
Gene count measurements were obtained directly from RSEM algorithm
Assembly: mm10
Supplementary files format and content: The processed data files are in tabular format, which contains gene_id, transcript_id(s), gene length, effective_length, expected_count, TPM, and FPKM
 
Submission date Feb 29, 2024
Last update date Apr 12, 2024
Contact name Yidong Chen
E-mail(s) cheny8@uthscsa.edu
Phone 2105629163
Organization name UT Health Science Center at San Antonio
Department Population Health Sciences
Street address 8403 Floyd Curl Drive, MSC 7784
City San Antonio
State/province Texas
ZIP/Postal code 78229
Country USA
 
Platform ID GPL24247
Series (1)
GSE260582 Mitochondrial ACSS1-K-635 acetylation mimic knock-in mice exhibit altered lipid metabolism, hepatic cell senescence, and acute non-alcoholic fatty liver [2ndSet_analysis]
Relations
BioSample SAMN40202809
SRA SRX23795152

Supplementary file Size Download File type/resource
GSM8119707_RSEM_228.genes.results.gz 429.7 Kb (ftp)(http) RESULTS
SRA Run SelectorHelp
Raw data are available in SRA

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