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Sample GSM8116300 Query DataSets for GSM8116300
Status Public on Dec 04, 2024
Title THP-1 cells, acetylated, 45 min, 2
Sample type SRA
 
Source name THP-1
Organism Homo sapiens
Characteristics cell line: THP-1
cell type: monocytic leukemia
genotype: WT
treatment: catalyst and acetyl donor
time: 45 min
Extracted molecule polyA RNA
Extraction protocol RNA was harvested using NucleoSpin RNA and reverse transcribed using SuperScript III First-Strand Synthesis System for RT-PCR (Invitrogen).
Libraries for RNA-seq were prepared using the TruSeq Stranded mRNA Sample Prep Kit (Illumina).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing Basecalls were performed using bcl2fastq.
Sequenced reads from RNA-seq experiments were aligned by using HISAT2 v2.1.0.
FPKM was calculated using cufflinks v2.2.1
Assembly: hg19
Supplementary files format and content: fpkm_tracking
 
Submission date Feb 28, 2024
Last update date Dec 04, 2024
Contact name Masaki Fukuyo
E-mail(s) fukuyo@chiba-u.jp
Organization name Chiba University
Department Department of Molecular Oncology
Street address 1-8-1 Inohana, Chuo-ku
City Chiba
ZIP/Postal code 260-8670
Country Japan
 
Platform ID GPL24676
Series (2)
GSE259436 Cancer epigenome intervention by in-cell chemical catalysis for histone modification (RNA-seq)
GSE259438 Cancer epigenome intervention by in-cell chemical catalysis for histone modification
Relations
BioSample SAMN40184322
SRA SRX23776444

Supplementary file Size Download File type/resource
GSM8116300_acetylated_45min_2.fpkm_tracking.gz 815.0 Kb (ftp)(http) FPKM_TRACKING
SRA Run SelectorHelp
Raw data are available in SRA

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