|
Status |
Public on Dec 04, 2024 |
Title |
THP-1 cells, acetylated, 45 min, 1 |
Sample type |
SRA |
|
|
Source name |
THP-1
|
Organism |
Homo sapiens |
Characteristics |
cell line: THP-1 cell type: monocytic leukemia genotype: WT treatment: catalyst and acetyl donor time: 45 min
|
Extracted molecule |
polyA RNA |
Extraction protocol |
RNA was harvested using NucleoSpin RNA and reverse transcribed using SuperScript III First-Strand Synthesis System for RT-PCR (Invitrogen). Libraries for RNA-seq were prepared using the TruSeq Stranded mRNA Sample Prep Kit (Illumina).
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Basecalls were performed using bcl2fastq. Sequenced reads from RNA-seq experiments were aligned by using HISAT2 v2.1.0. FPKM was calculated using cufflinks v2.2.1 Assembly: hg19 Supplementary files format and content: fpkm_tracking
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|
|
Submission date |
Feb 28, 2024 |
Last update date |
Dec 04, 2024 |
Contact name |
Masaki Fukuyo |
E-mail(s) |
fukuyo@chiba-u.jp
|
Organization name |
Chiba University
|
Department |
Department of Molecular Oncology
|
Street address |
1-8-1 Inohana, Chuo-ku
|
City |
Chiba |
ZIP/Postal code |
260-8670 |
Country |
Japan |
|
|
Platform ID |
GPL24676 |
Series (2) |
GSE259436 |
Cancer epigenome intervention by in-cell chemical catalysis for histone modification (RNA-seq) |
GSE259438 |
Cancer epigenome intervention by in-cell chemical catalysis for histone modification |
|
Relations |
BioSample |
SAMN40184326 |
SRA |
SRX23776440 |