|
| Status |
Public on Jun 20, 2024 |
| Title |
ATAC-Seq bone marrow immature B cells Ikzf1(AID/AID) R26(Tir2/Tir2) 8h 5-Ph-IAA_2 |
| Sample type |
SRA |
| |
|
| Source name |
bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: bone marrow
cell type: immature B cells
genotype: Ikzf1(AID/AID) R26(Tir2/Tir2)
cell preparation: Ex-vivo FACS sorted from bone marrow
treatment: 8h 5-Ph-IAA
replicate number: 2
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
King Fisher RNA isolation, rRNA depletion
ATAC-Seq libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
ATAC-seq |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
NextSeq 2000 |
| |
|
| Data processing |
ATAC-Seq: Only reads that passed the Illumina quality filtering were processed. Reads were aligned with the bowtie program version 2.3.5.1 to the mm10 genome version of December 2011 (GRCm38), including NZ_CP083274.1 Escherichia coli strain Rosetta2-DE3 chromosome (4.5Mb).
Assembly: mm10
Supplementary files format and content: bw (bigwig) = read coverage (reads per million) in bigWIG format
|
| |
|
| Submission date |
Feb 27, 2024 |
| Last update date |
Jun 20, 2024 |
| Contact name |
Meinrad Busslinger |
| E-mail(s) |
meinrad.busslinger@imp.ac.at
|
| Organization name |
IMP
|
| Lab |
Busslinger
|
| Street address |
Campus-Vienna-Biocenter 1
|
| City |
Vienna |
| ZIP/Postal code |
A-1030 |
| Country |
Austria |
| |
|
| Platform ID |
GPL30172 |
| Series (2) |
| GSE259355 |
Transcriptional function of E2A, Ebf1, Pax5, Ikaros and Aiolos analyzed by in vivo acute protein degradation in early B cell development [ATAC-seq] |
| GSE260523 |
Transcriptional function of E2A, Ebf1, Pax5, Ikaros and Aiolos analyzed by in vivo acute protein degradation in early B cell development |
|
| Relations |
| BioSample |
SAMN40160807 |
| SRA |
SRX23761462 |
