ATCC cell line <a href="http://www.atcc.org/SearchCatalogs/longview.cfm?view=ce,446344,CCL-247&text=CCL%2D247">CCL-247</a>. Colorectal carcinoma in a male. The cells are positive for keratin by immunoperoxidase staining. HCT 116 cells are positive for transforming growth factor beta 1 (TGF beta 1) and beta 2 (TGF beta 2) expression. This line has a mutation in codon 13 of the ras protooncogene, and can be used as a positive control for PCR assays of mutation in this codon.