|
| Status |
Public on Mar 15, 2024 |
| Title |
PBS-2. IP |
| Sample type |
SRA |
| |
|
| Source name |
lung
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lung
cell type: primary airway epithelial cells
genotype: WT
treatment: PBS
fraction: IP
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mouse AECs were isolated by fluorescence-activated cell sorting (FACS) using CD326-FITC (Thermo Fisher scientific), CD45-APC (BD Biosciences). Total mRNA was extracted from mouse AECs
m6A-Seq:TruSeq Stranded mRNA Sample Pre Kit (Illumina) was used to construct the library from immunoprecipitated mRNA and input mRNA following to a published protocol. mRNA-Seq:RNA libraries for RNA-Seq were preapred using SMARTER-Seq Library Prep Kit following manufatures's protocols.
m6a-seq, mRNA-seq
|
| |
|
| Library strategy |
RIP-Seq |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Differentially_methylated_sites.mRNA.xlsx
|
| Data processing |
cutadapt software, CLC Genomics Workbench v11.0.1
Differential m6A site information, Fold change, and P-value information identified by diffReps software.
Assembly: mm10
Supplementary files format and content: Differentially_methylated_sites.mRNA.xlsx
|
| |
|
| Submission date |
Feb 23, 2024 |
| Last update date |
Mar 15, 2024 |
| Contact name |
Jing Wang |
| E-mail(s) |
crystal2310@126.com
|
| Phone |
18930830739
|
| Organization name |
瑞金医院
|
| Street address |
huangpu Distinc, Ruijin Hospital
|
| City |
Shanghai |
| State/province |
Select... |
| ZIP/Postal code |
20000 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE256458 |
YTHDF1-CLOCK axis contributes to pathogenesis of allergic airway inflammation through LLPS [m6A-seq] |
|
| Relations |
| BioSample |
SAMN40077905 |
| SRA |
SRX23716732 |
