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Sample GSM8098146 Query DataSets for GSM8098146
Status Public on Jun 01, 2024
Title Cre+ 5d Bleo treatment
Sample type SRA
Source name skin
Organism Mus musculus
Characteristics tissue: skin
strain: AdiCreER; mTmG; Atgl fl/fl
age: 6-8 weeks
genotype: Cre+, Atgl fl/fl
treatment: Bleomycin
Treatment protocol AdiCreER; mT/mG; Atgl fl/fl male mice between 6-8 weeks are shaved and receive 1 dose of topical tamoxifen (5mg/ml in 100% ethanol) on the back skin 2 days prior to injections. Mice were then injected subcutaneously for 5 days with either 100ul of 300ug/ml PBS or Bleomycin Sulfate. Back skin was collected and dissociated and submitted for scRNA-sequencing.
Extracted molecule total RNA
Extraction protocol Mouse skin was manually dissociated with scissors and then enzymatically with Liberase TL dissociated in a 37C shaking water minutes for 30minutes. The enzymatic reaction was halted. Cells were filtered through a 40um mesh.
Library was performed by the manufacturers instructions
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Description 10x Genomics
Data processing The demultiplexing, barcoding, gene counting and aggregation were made using the Cell Ranger v2.1.1
Assembly: mm10
Supplementary files format and content: Cell-Ranger filtered_feature h5 files
Submission date Feb 22, 2024
Last update date Jun 01, 2024
Contact name Elizabeth Ann Caves
Organization name Yale University
Department Molecular, Cellular and Developmental Biology
Lab Horsley Lab
Street address 260 Whitney Ave
City New Haven
State/province Connecticut
ZIP/Postal code 06511
Country USA
Platform ID GPL24247
Series (1)
GSE256411 The role of adipocyte lipolysis during dermal fibrosis
BioSample SAMN40041708
SRA SRX23706302

Supplementary file Size Download File type/resource
GSM8098146_11AGABleo_filtered_feature_bc_matrix.h5 1.1 Mb (ftp)(http) H5
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Raw data are available in SRA

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