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Sample GSM8093340 Query DataSets for GSM8093340
Status Public on Feb 26, 2024
Title Liver, Mir483Pat-KO, rep1 (RNA-seq)
Sample type SRA
 
Source name Liver
Organism Mus musculus
Characteristics tissue: Liver
Sex: female
developmental stage: E18.5
genotype: Mir483Pat-KO
index: SLX-22976.UDI0060rna
Extracted molecule polyA RNA
Extraction protocol Total RNA was extracted using a miRNeasy Mini Kit (Qiagen – 217004), according to manufacturer's protocol. Quantity and quality of RNA were assessed using an Agilent 2100 Bioanalyzer and RNA 6000 Nano Kits (Agilent – 5067-1511)
500 ng total RNA per sample was used to prepare libraries using Illumina's TruSeq RNA Library kit, according to manufacturer's protocol.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description Liver, Mir483Pat-KO, rep1 miR483_426
Data processing STAR 2.5.0A was used to perform the alignment and gene level count with GRCm38/Ensembl 100
Assembly: GRCm38
Supplementary files format and content: Gene level count in tab-delimited format
 
Submission date Feb 21, 2024
Last update date Feb 26, 2024
Contact name Brian Yee Hong Lam
E-mail(s) yhbl2@cam.ac.uk
Organization name University of Cambridge
Department Metabolic Research Laboratories
Street address Box 289 Addenbookes Hospital, Hills Road
City Cambridge
ZIP/Postal code CB2 0QQ
Country United Kingdom
 
Platform ID GPL24247
Series (1)
GSE256302 Expression profiling of mouse livers with deletion of paternally-expressed, imprinted Mir483 [RNA-seq]
Relations
BioSample SAMN40024832
SRA SRX23689650

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA

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