|
| Status |
Public on Feb 19, 2024 |
| Title |
control |
| Sample type |
SRA |
| |
|
| Source name |
lung
|
| Organism |
Mus musculus x Rattus norvegicus |
| Characteristics |
tissue: lung
strain: C57BL/6, SD
age: E20.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The lung samples were harvested from E20.5 mouse-rat chimeric embryo. The samples were dissociated into small pieces and digested into single cell suspension.
The libraries were prepared for sequencing using standard 10X 3'v3 protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
10x Genomics
|
| Data processing |
The standard celllranger mkfastq software version 3.0 used for basecalling.
The standard celllranger count software version 3.0 used to produced raw expression matrix.
The matrix were normalized and scaled by Seurat v4.3 in R4.2.1 environment.
Assembly: mm10,rn6
Supplementary files format and content: Tab-separated values files and matrix files
|
| |
|
| Submission date |
Feb 16, 2024 |
| Last update date |
Feb 19, 2024 |
| Contact name |
Enhong Li |
| E-mail(s) |
lienhong0808@gmail.com
|
| Organization name |
The University of Arizona
|
| Department |
Child Health
|
| Lab |
Kalinichenko Lab
|
| Street address |
475 N 5th st
|
| City |
PHOENIX |
| State/province |
AZ |
| ZIP/Postal code |
85004 |
| Country |
USA |
| |
|
| Platform ID |
GPL34201 |
| Series (1) |
| GSE255966 |
The single cell RNAseq analysis of regenerated mouse lung in mouse-rat interspecies |
|
| Relations |
| BioSample |
SAMN39967057 |
| SRA |
SRX23643524 |
