|
Status |
Public on Jul 05, 2024 |
Title |
Alveolar macrophages from control mouse (sample #1) |
Sample type |
SRA |
|
|
Source name |
Lung
|
Organism |
Mus musculus |
Characteristics |
tissue: Lung cell type: Primary alveolar macrophages genotype: Sftpc-CreERt2(+/-)
|
Extracted molecule |
total RNA |
Extraction protocol |
Cell as input and lysis accroding to manufacturer's instructions of SMART-Seq Stranded Kit (Takara) Libraries were prepared using the SMART-Seq Stranded Kit (Takara)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
N11301_01
|
Data processing |
Trimming adaptor sequences and removing reads of low quality were performed by cutadapt (v2.4). Quantified reads were aligned to the mouse genome (GRCm38) by STAR (v2.7.2a) and then gene-level read counts were generated based on the annotations of Gencode (vM25) Assembly: GRCm38 Supplementary files format and content: tab-delimited text files include read count values for each sample
|
|
|
Submission date |
Feb 16, 2024 |
Last update date |
Jul 05, 2024 |
Contact name |
Chia-Lang Hsu |
E-mail(s) |
chialanghsu@ntuh.gov.tw
|
Organization name |
National Taiwan University Hospital
|
Department |
Department of Medical Research
|
Street address |
No. 7, Zhongshan S. Rd.
|
City |
Taipei |
ZIP/Postal code |
100 |
Country |
Taiwan |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE255949 |
Gene expression of alveolar macrophage in mice with AT2 cell-specific Cpt1a deletion |
|
Relations |
BioSample |
SAMN39964386 |
SRA |
SRX23642287 |