|
| Status |
Public on Feb 10, 2024 |
| Title |
P83-P90 Acta2-CreERT2, R26R-LSL-EYFP |
| Sample type |
SRA |
| |
|
| Source name |
lung
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lung
genotype: Acta2-CreERT2, R26R-LSL-EYFP
treatment: P83 tamoxifen
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Whole mouse lung dissociated into single-cell suspension before FACS.
10X genomics GEXv3 3’-scRNA-seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Read alignment using STAR-solo
Ambient RNA correction using scvi-tools scAR model
QC, filtering, normalization using scanpy
Batch-correction using harmony-pytorch
Projections and clustering using scanpy
Assembly: GRCm39
Supplementary files format and content: anndata hdf5 (h5ad) gzipped Yap/Taz KO scRNA-seq dataset, processed using scanpy, containing cell barcodes, annotations, and gene expression values (raw and normalized).
Supplementary files format and content: compressed zip file of STAR-solo gene expression matrices for lineage trace experiments.
|
| |
|
| Submission date |
Feb 05, 2024 |
| Last update date |
Feb 10, 2024 |
| Contact name |
Sylvia N Michki |
| E-mail(s) |
michkin@chop.edu
|
| Organization name |
Children's Hospital of Philadelphia
|
| Department |
Pulmonary & Sleep Medicine
|
| Street address |
3615 Civic Center Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE255060 |
Dynamic Hippo pathway activity underlies mesenchymal differentiation during lung alveolar morphogenesis |
|
| Relations |
| BioSample |
SAMN39831114 |
| SRA |
SRX23534698 |
