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Sample GSM8063207 Query DataSets for GSM8063207
Status Public on Feb 10, 2024
Title P1-P23 Acta2-CreERT2, Yap/Taz fl/fl KO, rep2, CD45-
Sample type SRA
 
Source name lung
Organism Mus musculus
Characteristics tissue: lung
genotype: Acta2-CreERT2, R26R-LSL-EYFP, Yap/Taz fl/fl KO
treatment: P1 tamoxifen
Extracted molecule polyA RNA
Extraction protocol Whole mouse lung dissociated into single-cell suspension before FACS.
10X genomics GEXv3 3’-scRNA-seq
 
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing Read alignment using STAR-solo
Ambient RNA correction using scvi-tools scAR model
QC, filtering, normalization using scanpy
Batch-correction using harmony-pytorch
Projections and clustering using scanpy
Assembly: GRCm39
Supplementary files format and content: anndata hdf5 (h5ad) gzipped Yap/Taz KO scRNA-seq dataset, processed using scanpy, containing cell barcodes, annotations, and gene expression values (raw and normalized).
Supplementary files format and content: compressed zip file of STAR-solo gene expression matrices for lineage trace experiments.
 
Submission date Feb 05, 2024
Last update date Feb 10, 2024
Contact name Sylvia N Michki
E-mail(s) michkin@chop.edu
Organization name Children's Hospital of Philadelphia
Department Pulmonary & Sleep Medicine
Street address 3615 Civic Center Blvd
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL24247
Series (1)
GSE255060 Dynamic Hippo pathway activity underlies mesenchymal differentiation during lung alveolar morphogenesis
Relations
BioSample SAMN39831115
SRA SRX23534697

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA

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