|
| Status |
Public on Feb 05, 2024 |
| Title |
shNC-D2-rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Liver
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
genotype: C57BL/6J
treatment: shNC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from mouse liver using TRIzol (MRC, RN190) following the manufacturer’s instructions.
Bulk RNA-seq libraries were prepared using MGIEasy RNA Library Prep Set (MGI, 1000006384) according to the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
MGISEQ-2000RS |
| |
|
| Data processing |
RNA-seq data was mapped to mm10 (Ensembl 93) reference genome with STAR, and gene expression was quantified in an RSEM-based pipeline. DEGs were determined by DESeq2 (V1.30.1) and were defined as an absolute fold change > 1.5 and P value < 0.05.
Assembly: RNA-seq data was mapped to mm10 (Ensembl 93) reference genome with a STAR-based RSEM pipeline.
Supplementary files format and content: Count matrixes generated by RSEM.
|
| |
|
| Submission date |
Jan 29, 2024 |
| Last update date |
Feb 05, 2024 |
| Contact name |
Yiwei Lai |
| E-mail(s) |
lai_yiwei@gibh.ac.cn
|
| Organization name |
Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences
|
| Department |
South China Institute for Stem Cell Biology and Regenerative Medicine Key Laboratory of Regenerative Biology, CAS
|
| Lab |
Miguel
|
| Street address |
190 Kai Yuan Avenue, Science Park
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
|
| Platform ID |
GPL30215 |
| Series (1) |
| GSE254481 |
Effect of knockdown Tbl1xr1 on gene expression after 2/3 hepatectomy |
|
| Relations |
| BioSample |
SAMN39657155 |
| SRA |
SRX23429149 |
