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Sample GSM803527 Query DataSets for GSM803527
Status Public on Sep 29, 2011
Title HudsonAlpha_ChipSeq_HepG2_USF-1_PCR1x
Sample type SRA
Source name HepG2
Organism Homo sapiens
Characteristics datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell description: hepatocellular carcinoma
antibody antibodydescription: Rabbit polyclonal, USF-1 (C-20). Antibody Target: USF1
antibody targetdescription: The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of USF-1 and USF-2 polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an up- stream stimulatory factor that binds the core sequence CACGTG in the adeno- virus late promoter. These findings, together with the demonstration of coop- erative interaction between USF and the initiator-binding protein, TFII-I, raises the possibility of a more general involvement of USF in transcriptional regula- tion. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types.
antibody vendorname: Santa Cruz Biotechnology
antibody vendorid: sc-229
protocol description: one 15-cycle round of PCR (Myers)
controlid: SL819,SL593
labexpid: SL636,SL582
labversion: SL636,SL582
replicate: 1,2
softwareversion: MACS
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-229
treatment: None
treatment description: No special treatment or protocol applies
protocol: PCR1x
protocol description: 1-cycle of PCR (Myers)
controlid: SL593
labexpid: SL582
replicate: 1
replicate description: tier 1
Biomaterial provider ATCC
Treatment protocol None
Growth protocol HepG2_protocol.pdf
Extracted molecule genomic DNA
Extraction protocol Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer
Data processing
Submission date Sep 28, 2011
Last update date May 15, 2019
Contact name ENCODE DCC
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
Platform ID GPL9052
Series (2)
GSE32465 Transcription Factor Binding Sites by ChIP-seq from ENCODE/HAIB
GSE51334 DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions
SRA SRX100572
BioSample SAMN00738489
Named Annotation GSM803527_hg19_wgEncodeHaibTfbsHepg2Usf1Pcr1xRawRep1.bigWig
Named Annotation GSM803527_hg19_wgEncodeHaibTfbsHepg2Usf1Pcr1xRawRep2.bigWig

Supplementary file Size Download File type/resource
GSM803527_hg19_wgEncodeHaibTfbsHepg2Usf1Pcr1xPkRep1.broadPeak.gz 311.7 Kb (ftp)(http) BROADPEAK
GSM803527_hg19_wgEncodeHaibTfbsHepg2Usf1Pcr1xPkRep2.broadPeak.gz 607.2 Kb (ftp)(http) BROADPEAK
GSM803527_hg19_wgEncodeHaibTfbsHepg2Usf1Pcr1xRawRep1.bigWig 13.0 Mb (ftp)(http) BIGWIG
GSM803527_hg19_wgEncodeHaibTfbsHepg2Usf1Pcr1xRawRep2.bigWig 56.7 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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