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Sample GSM803493 Query DataSets for GSM803493
Status Public on Sep 29, 2011
Title HudsonAlpha_ChipSeq_HepG2_HDAC2_(SC-6296)_v041610.1
Sample type SRA
 
Source name HepG2
Organism Homo sapiens
Characteristics datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell description: hepatocellular carcinoma
antibody antibodydescription: Goat polyclonal IgG, epitope mapping at the C-terminus of HDAC2 of human origin. Antibody Target: HDAC2
antibody targetdescription: This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. (provided by RefSeq)
antibody vendorname: Santa Cruz Biotechnology
antibody vendorid: sc-6296
controlid: SL1781
labexpid: SL3177,SL3583
replicate: 1,2
softwareversion: MACS
antibody antibodydescription: Goat polyclonal IgG, epitope mapping at the C-terminus of HDAC2 of human origin.
antibody targetdescription: This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. (provided by RefSeq)
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-6296
treatment: None
treatment description: No special treatment or protocol applies
protocol: v041610.1
protocol description: 2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
controlid: SL1781
labexpid: SL3177
replicate: 1
replicate description: tier 1
Biomaterial provider ATCC
Treatment protocol None
Growth protocol HepG2_protocol.pdf
Extracted molecule genomic DNA
Extraction protocol Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer
 
Data processing http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
 
Submission date Sep 28, 2011
Last update date May 15, 2019
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL9052
Series (2)
GSE32465 Transcription Factor Binding Sites by ChIP-seq from ENCODE/HAIB
GSE51334 DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions
Relations
SRA SRX100538
BioSample SAMN00738455
Named Annotation GSM803493_hg19_wgEncodeHaibTfbsHepg2Hdac2sc6296V0416101RawRep1.bigWig
Named Annotation GSM803493_hg19_wgEncodeHaibTfbsHepg2Hdac2sc6296V0416101RawRep2.bigWig

Supplementary file Size Download File type/resource
GSM803493_hg19_wgEncodeHaibTfbsHepg2Hdac2sc6296V0416101PkRep1.broadPeak.gz 503.1 Kb (ftp)(http) BROADPEAK
GSM803493_hg19_wgEncodeHaibTfbsHepg2Hdac2sc6296V0416101PkRep2.broadPeak.gz 115.3 Kb (ftp)(http) BROADPEAK
GSM803493_hg19_wgEncodeHaibTfbsHepg2Hdac2sc6296V0416101RawRep1.bigWig 41.0 Mb (ftp)(http) BIGWIG
GSM803493_hg19_wgEncodeHaibTfbsHepg2Hdac2sc6296V0416101RawRep2.bigWig 15.9 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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