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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 29, 2011 |
| Title |
HudsonAlpha_ChipSeq_K562_E2F6_v041610.2 |
| Sample type |
SRA |
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| Source name |
K562
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| Organism |
Homo sapiens |
| Characteristics |
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
antibody antibodydescription: Rabbit polyclonal IgG, epitope corresponding to a.a. 232-281 mapping at the C-terminus of E2F-6 of human origin. Antibody Target: E2F6
antibody targetdescription: This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms (RefSeq).
antibody vendorname: Santa Cruz Biotechnology
antibody vendorid: sc-22823
controlid: SL1396,SL1397
labexpid: SL3073,SL2948
replicate: 1,2
softwareversion: MACS
antibody: E2F6
antibody antibodydescription: Rabbit polyclonal IgG, epitope corresponding to a.a. 232-281 mapping at the C-terminus of E2F-6 of human origin.
antibody targetdescription: This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms (RefSeq).
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-22823
treatment: None
treatment description: No special treatment or protocol applies
protocol: v041610.2
protocol description: 2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid: SL1396
labexpid: SL2948
replicate: 1
replicate description: tier 1
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| Biomaterial provider |
ATCC
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| Treatment protocol |
None
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| Growth protocol |
K562_protocol.pdf
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
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| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
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| Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
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| Submission date |
Sep 28, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
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| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
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| Platform ID |
GPL9052 |
| Series (2) |
| GSE32465 |
Transcription Factor Binding Sites by ChIP-seq from ENCODE/HAIB |
| GSE51334 |
DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions |
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| Relations |
| SRA |
SRX100514 |
| BioSample |
SAMN00738431 |
| Named Annotation |
GSM803469_hg19_wgEncodeHaibTfbsK562E2f6sc22823V0416102RawRep1.bigWig |
| Named Annotation |
GSM803469_hg19_wgEncodeHaibTfbsK562E2f6sc22823V0416102RawRep2.bigWig |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM803469_hg19_wgEncodeHaibTfbsK562E2f6sc22823V0416102PkRep1.broadPeak.gz |
224.8 Kb |
(ftp)(http) |
BROADPEAK |
| GSM803469_hg19_wgEncodeHaibTfbsK562E2f6sc22823V0416102PkRep2.broadPeak.gz |
643.4 Kb |
(ftp)(http) |
BROADPEAK |
| GSM803469_hg19_wgEncodeHaibTfbsK562E2f6sc22823V0416102RawRep1.bigWig |
17.0 Mb |
(ftp)(http) |
BIGWIG |
| GSM803469_hg19_wgEncodeHaibTfbsK562E2f6sc22823V0416102RawRep2.bigWig |
41.8 Mb |
(ftp)(http) |
BIGWIG |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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