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Sample GSM803438 Query DataSets for GSM803438
Status Public on Sep 29, 2011
Title HudsonAlpha_ChipSeq_H1-hESC_POU5F1_(SC-9081)_v041610.2
Sample type SRA
 
Source name H1-hESC
Organism Homo sapiens
Characteristics datatype: ChipSeq
datatype description: Chromatin IP Sequencing
antibody antibodydescription: Rabbit polyclonal IgG, epitope corresponding to amino acids 1-134 of Oct3/4 of human origin. Antibody Target: POU5F1
antibody targetdescription: This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12 (provided by RefSeq).
antibody vendorname: Santa Cruz Biotechnology
antibody vendorid: sc-9081
controlid: SL1398
labexpid: SL2548,SL3453
replicate: 1,2
softwareversion: MACS
antibody: POU5F1_(SC-9081)
antibody antibodydescription: Rabbit polyclonal IgG, epitope corresponding to amino acids 1-134 of Oct3/4 of human origin.
antibody targetdescription: This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12 (provided by RefSeq).
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-9081
treatment: None
treatment description: No special treatment or protocol applies
protocol: v041610.2
protocol description: 2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid: SL1398
labexpid: SL2548
replicate: 1
replicate description: tier 1
Biomaterial provider WiCell Research Institute
Treatment protocol None
Growth protocol missing
Extracted molecule genomic DNA
Extraction protocol Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer
 
Data processing http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
 
Submission date Sep 28, 2011
Last update date May 15, 2019
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL9052
Series (2)
GSE32465 Transcription Factor Binding Sites by ChIP-seq from ENCODE/HAIB
GSE51334 DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions
Relations
SRA SRX100483
BioSample SAMN00738400
Named Annotation GSM803438_hg19_wgEncodeHaibTfbsH1hescPou5f1sc9081V0416102RawRep1.bigWig
Named Annotation GSM803438_hg19_wgEncodeHaibTfbsH1hescPou5f1sc9081V0416102RawRep2.bigWig

Supplementary file Size Download File type/resource
GSM803438_hg19_wgEncodeHaibTfbsH1hescPou5f1sc9081V0416102PkRep1.broadPeak.gz 49.8 Kb (ftp)(http) BROADPEAK
GSM803438_hg19_wgEncodeHaibTfbsH1hescPou5f1sc9081V0416102PkRep2.broadPeak.gz 26.9 Kb (ftp)(http) BROADPEAK
GSM803438_hg19_wgEncodeHaibTfbsH1hescPou5f1sc9081V0416102RawRep1.bigWig 3.8 Mb (ftp)(http) BIGWIG
GSM803438_hg19_wgEncodeHaibTfbsH1hescPou5f1sc9081V0416102RawRep2.bigWig 15.0 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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