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Sample GSM803437 Query DataSets for GSM803437
Status Public on Sep 29, 2011
Title HudsonAlpha_ChipSeq_H1-hESC_NANOG_(SC-33759)_v041610.2
Sample type SRA
 
Source name H1-hESC
Organism Homo sapiens
Characteristics datatype: ChipSeq
datatype description: Chromatin IP Sequencing
antibody antibodydescription: Rabbit polyclonal IgG, epitope corresponding to amino acids 151-305 mapping at the C-terminus of Nanog of human origin. Antibody Target: NANOG
antibody targetdescription: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity) (provided by RefSeq).
antibody vendorname: Santa Cruz Biotechnology
antibody vendorid: sc-33759
controlid: SL1398
labexpid: SL2687,SL3068
replicate: 1,2
softwareversion: MACS
antibody: NANOG_(SC-33759)
antibody antibodydescription: Rabbit polyclonal IgG, epitope corresponding to amino acids 151-305 mapping at the C-terminus of Nanog of human origin.
antibody targetdescription: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity) (provided by RefSeq).
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-33759
treatment: None
treatment description: No special treatment or protocol applies
protocol: v041610.2
protocol description: 2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid: SL1398
labexpid: SL2687
replicate: 1
replicate description: tier 1
Biomaterial provider WiCell Research Institute
Treatment protocol None
Growth protocol missing
Extracted molecule genomic DNA
Extraction protocol Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer
 
Data processing http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
 
Submission date Sep 28, 2011
Last update date May 15, 2019
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL9052
Series (2)
GSE32465 Transcription Factor Binding Sites by ChIP-seq from ENCODE/HAIB
GSE51334 DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions
Relations
SRA SRX100482
BioSample SAMN00738399
Named Annotation GSM803437_hg19_wgEncodeHaibTfbsH1hescNanogsc33759V0416102RawRep1.bigWig
Named Annotation GSM803437_hg19_wgEncodeHaibTfbsH1hescNanogsc33759V0416102RawRep2.bigWig

Supplementary file Size Download File type/resource
GSM803437_hg19_wgEncodeHaibTfbsH1hescNanogsc33759V0416102PkRep1.broadPeak.gz 73.5 Kb (ftp)(http) BROADPEAK
GSM803437_hg19_wgEncodeHaibTfbsH1hescNanogsc33759V0416102PkRep2.broadPeak.gz 207.9 Kb (ftp)(http) BROADPEAK
GSM803437_hg19_wgEncodeHaibTfbsH1hescNanogsc33759V0416102RawRep1.bigWig 2.1 Mb (ftp)(http) BIGWIG
GSM803437_hg19_wgEncodeHaibTfbsH1hescNanogsc33759V0416102RawRep2.bigWig 50.4 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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