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Sample GSM803401 Query DataSets for GSM803401
Status Public on Sep 29, 2011
Title HudsonAlpha_ChipSeq_K562_CTCFL_(SC-98982)_v041610.1
Sample type SRA
 
Source name K562
Organism Homo sapiens
Characteristics datatype: ChipSeq
datatype description: Chromatin IP Sequencing
antibody antibodydescription: Rabbit polyclonal IgG, epitope corresponding to amino acids 91-210 mapping within an internal region of BORIS(CTCFL) of human origin. Antibody Target: CTCFL
antibody targetdescription: CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. (provided by RefSeq)
antibody vendorname: Santa Cruz Biotechnology
antibody vendorid: sc-98982
controlid: SL3980,SL2455
labexpid: SL3189,SL3972
replicate: 1,2
softwareversion: MACS
antibody: CTCFL_(SC-98982)
antibody antibodydescription: Rabbit polyclonal IgG, epitope corresponding to amino acids 91-210 mapping within an internal region of BORIS(CTCFL) of human origin.
antibody targetdescription: CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. (provided by RefSeq)
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-98982
treatment: None
treatment description: No special treatment or protocol applies
protocol: v041610.1
protocol description: 2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
controlid: SL2455
labexpid: SL3189
replicate: 1
replicate description: tier 1
Biomaterial provider ATCC
Treatment protocol None
Growth protocol K562_protocol.pdf
Extracted molecule genomic DNA
Extraction protocol Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer
 
Data processing http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
 
Submission date Sep 28, 2011
Last update date May 15, 2019
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL9052
Series (2)
GSE32465 Transcription Factor Binding Sites by ChIP-seq from ENCODE/HAIB
GSE51334 DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions
Relations
SRA SRX100446
BioSample SAMN00738363
Named Annotation GSM803401_hg19_wgEncodeHaibTfbsK562Ctcflsc98982V0416101RawRep1.bigWig
Named Annotation GSM803401_hg19_wgEncodeHaibTfbsK562Ctcflsc98982V0416101RawRep2.bigWig

Supplementary file Size Download File type/resource
GSM803401_hg19_wgEncodeHaibTfbsK562Ctcflsc98982V0416101PkRep1.broadPeak.gz 130.1 Kb (ftp)(http) BROADPEAK
GSM803401_hg19_wgEncodeHaibTfbsK562Ctcflsc98982V0416101PkRep2.broadPeak.gz 200.5 Kb (ftp)(http) BROADPEAK
GSM803401_hg19_wgEncodeHaibTfbsK562Ctcflsc98982V0416101RawRep1.bigWig 18.8 Mb (ftp)(http) BIGWIG
GSM803401_hg19_wgEncodeHaibTfbsK562Ctcflsc98982V0416101RawRep2.bigWig 15.1 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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