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Sample GSM803377 Query DataSets for GSM803377
Status Public on Sep 29, 2011
Title HudsonAlpha_ChipSeq_H1-hESC_SP1_PCR1x
Sample type SRA
 
Source name H1-hESC
Organism Homo sapiens
Characteristics datatype: ChipSeq
datatype description: Chromatin IP Sequencing
antibody antibodydescription: Sp1(E-3) is a mouse monoclonal antibody raised against amino acids 121-345 mapping near the N-terminus of Sp1 ofhuman origin. Recommended for detection of Sp1 of m, r and h origin by WB, IP, IF and ELISA. Antibody Target: SP1
antibody targetdescription: SP1 is a transcription factor that binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. It can interact with G/C-rich motifs from the serotonin receptor promoter.
antibody vendorname: Santa Cruz Biotechnology
antibody vendorid: sc-17824
protocol description: one 15-cycle round of PCR (Myers)
controlid: SL1398
labexpid: SL1318,SL1166
replicate: 1,2
softwareversion: MACS
antibody antibodydescription: Sp1(E-3) is a mouse monoclonal antibody raised against amino acids 121-345 mapping near the N-terminus of Sp1 ofhuman origin. Recommended for detection of Sp1 of m, r and h origin by WB, IP, IF and ELISA.
antibody targetdescription: SP1 is a transcription factor that binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. It can interact with G/C-rich motifs from the serotonin receptor promoter.
antibody vendorname: Santa Cruz Biotech
antibody vendorid: sc-17824
treatment: None
treatment description: No special treatment or protocol applies
protocol: PCR1x
protocol description: 1-cycle of PCR (Myers)
controlid: SL1398
labexpid: SL1166
replicate: 1
replicate description: tier 1
Biomaterial provider WiCell Research Institute
Treatment protocol None
Growth protocol missing
Extracted molecule genomic DNA
Extraction protocol Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer
 
Data processing http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
 
Submission date Sep 28, 2011
Last update date May 15, 2019
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL9052
Series (2)
GSE32465 Transcription Factor Binding Sites by ChIP-seq from ENCODE/HAIB
GSE51334 DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions
Relations
SRA SRX100422
BioSample SAMN00738339
Named Annotation GSM803377_hg19_wgEncodeHaibTfbsH1hescSp1Pcr1xRawRep1.bigWig
Named Annotation GSM803377_hg19_wgEncodeHaibTfbsH1hescSp1Pcr1xRawRep2.bigWig

Supplementary file Size Download File type/resource
GSM803377_hg19_wgEncodeHaibTfbsH1hescSp1Pcr1xPkRep1.broadPeak.gz 280.1 Kb (ftp)(http) BROADPEAK
GSM803377_hg19_wgEncodeHaibTfbsH1hescSp1Pcr1xPkRep2.broadPeak.gz 257.0 Kb (ftp)(http) BROADPEAK
GSM803377_hg19_wgEncodeHaibTfbsH1hescSp1Pcr1xRawRep1.bigWig 10.3 Mb (ftp)(http) BIGWIG
GSM803377_hg19_wgEncodeHaibTfbsH1hescSp1Pcr1xRawRep2.bigWig 10.5 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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