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Sample GSM8027353 Query DataSets for GSM8027353
Status Public on Apr 02, 2024
Title Young normal _rep1
Sample type RNA
 
Source name Peripheral Blood
Organism Homo sapiens
Characteristics tissue: plasma
gender: male
age: 39y
individual: Young normal
Treatment protocol Plasma was collected from blood anti-coagulated with ethylene diamine tetraaceticacid (EDTA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRIzol (Invitrogen) and miRNeasy mini kit (QIAGEN) according to manufacturer’s instructions, which efficiently recovered all RNA species, including miRNAs. RNA quality and quantity was measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis
Label Hy3
Label protocol The miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) was used according to the manufacturer’s guideline for miRNA labelling
 
Hybridization protocol The Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.16.0) (Exiqon) according to array manual
Scan protocol The slides were scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).
Description miRNA expression of human plasma
Data processing Scanned images were then imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction. Replicated miRNAs were averaged and miRNAs that intensities>=50 in all samples were chosen for calculating normalization factor. Expressed data were normalized using the Median normalization. After normalization, differentially expressed miRNAs with statistical significance were identified through Volcano Plot filtering
 
Submission date Jan 19, 2024
Last update date Apr 02, 2024
Contact name Jingsong Ou
E-mail(s) oujs@mail.sysu.edu.cn
Phone 862087755766-8238
Organization name The First Affiliated Hospital, Sun Yat-sen University
Department Surgery/Cardiac Surgery
Street address 58 Zhong Shan Er Road
City Guangzhou
State/province Please Select
ZIP/Postal code 510080
Country China
 
Platform ID GPL21439
Series (1)
GSE253747 Aging aggravates aortic aneurysm and dissection via the miR-1204-MYLK signaling axis

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
13138 0.014727541
42638 0.546391753
42888 3.913107511
17519
17278 0.515463918
46507 0.28718704
17928 0.032400589
42826 0.711340206
17537 0.390279823
42722 0.346097202
42645
46636
11134 3.755522828
17295 1.083946981
32825 2.462444772
46276
42812 0.107511046
42918 0.086892489
46457 0.157584683
42469 0.011782032

Total number of rows: 3556

Table truncated, full table size 62 Kbytes.




Supplementary file Size Download File type/resource
GSM8027353_Young_normal_1.gpr.gz 968.2 Kb (ftp)(http) GPR

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