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Sample GSM8027352 Query DataSets for GSM8027352
Status Public on Apr 02, 2024
Title Elder patient _rep3
Sample type RNA
 
Source name Peripheral Blood
Organism Homo sapiens
Characteristics tissue: plasma
gender: male
age: 78y
individual: Elder patient
Treatment protocol Plasma was collected from blood anti-coagulated with ethylene diamine tetraaceticacid (EDTA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRIzol (Invitrogen) and miRNeasy mini kit (QIAGEN) according to manufacturer’s instructions, which efficiently recovered all RNA species, including miRNAs. RNA quality and quantity was measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis
Label Hy3
Label protocol The miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) was used according to the manufacturer’s guideline for miRNA labelling
 
Hybridization protocol The Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.16.0) (Exiqon) according to array manual
Scan protocol The slides were scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).
Description miRNA expression of human plasma
Data processing Scanned images were then imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction. Replicated miRNAs were averaged and miRNAs that intensities>=50 in all samples were chosen for calculating normalization factor. Expressed data were normalized using the Median normalization. After normalization, differentially expressed miRNAs with statistical significance were identified through Volcano Plot filtering
 
Submission date Jan 19, 2024
Last update date Apr 02, 2024
Contact name Jingsong Ou
E-mail(s) oujs@mail.sysu.edu.cn
Phone 862087755766-8238
Organization name The First Affiliated Hospital, Sun Yat-sen University
Department Surgery/Cardiac Surgery
Street address 58 Zhong Shan Er Road
City Guangzhou
State/province Please Select
ZIP/Postal code 510080
Country China
 
Platform ID GPL21439
Series (1)
GSE253747 Aging aggravates aortic aneurysm and dissection via the miR-1204-MYLK signaling axis

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
13138 0.029149798
42638 0.652631579
42888 6.103643725
17519 0.003238866
17278 1.251821862
46507 0.246153846
17928 0.110121457
42826 1.2
17537 0.427530364
42722 0.471255061
42645 0.027530364
46636
11134 5.732793522
17295 1.480161943
32825 3.891497976
46276 0.001619433
42812 0.168421053
42918 0.066396761
46457 0.396761134
42469 0.025910931

Total number of rows: 3556

Table truncated, full table size 61 Kbytes.




Supplementary file Size Download File type/resource
GSM8027352_Elder_patient_3.gpr.gz 996.3 Kb (ftp)(http) GPR

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