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Sample GSM8027349 Query DataSets for GSM8027349
Status Public on Apr 02, 2024
Title Young patient _rep3
Sample type RNA
 
Source name Peripheral Blood
Organism Homo sapiens
Characteristics tissue: plasma
gender: male
age: 43y
individual: Young patient
Treatment protocol Plasma was collected from blood anti-coagulated with ethylene diamine tetraaceticacid (EDTA).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRIzol (Invitrogen) and miRNeasy mini kit (QIAGEN) according to manufacturer’s instructions, which efficiently recovered all RNA species, including miRNAs. RNA quality and quantity was measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis
Label Hy3
Label protocol The miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) was used according to the manufacturer’s guideline for miRNA labelling
 
Hybridization protocol The Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.16.0) (Exiqon) according to array manual
Scan protocol The slides were scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).
Description miRNA expression of human plasma
Data processing Scanned images were then imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction. Replicated miRNAs were averaged and miRNAs that intensities>=50 in all samples were chosen for calculating normalization factor. Expressed data were normalized using the Median normalization. After normalization, differentially expressed miRNAs with statistical significance were identified through Volcano Plot filtering
 
Submission date Jan 19, 2024
Last update date Apr 02, 2024
Contact name Jingsong Ou
E-mail(s) oujs@mail.sysu.edu.cn
Phone 862087755766-8238
Organization name The First Affiliated Hospital, Sun Yat-sen University
Department Surgery/Cardiac Surgery
Street address 58 Zhong Shan Er Road
City Guangzhou
State/province Please Select
ZIP/Postal code 510080
Country China
 
Platform ID GPL21439
Series (1)
GSE253747 Aging aggravates aortic aneurysm and dissection via the miR-1204-MYLK signaling axis

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
13138 0.006294256
42638 1.280881196
42888 3.590873328
17519
17278 1.326514555
46507 0.242328875
17928 0.061369001
42826 0.508261212
17537 0.838709677
42722 0.173092054
42645 0.006294256
46636 0.004720692
11134 4.791502754
17295 0.575924469
32825 3.18332022
46276 0.003147128
42812 0.086546027
42918 0.037765539
46457 0.129032258
42469 0.007867821

Total number of rows: 3556

Table truncated, full table size 63 Kbytes.




Supplementary file Size Download File type/resource
GSM8027349_Young_patient_3.gpr.gz 975.5 Kb (ftp)(http) GPR

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