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Sample GSM802368 Query DataSets for GSM802368
Status Public on Nov 30, 2011
Title High fat diet mouse2
Sample type RNA
 
Channel 1
Source name adipose tissue from high fat diet mouse
Organism Mus musculus
Characteristics tissue: Adipose tissue
Extracted molecule total RNA
Extraction protocol Total RNA extracted using guanidinium thiocyanate following manufacturer's instructions
Label Hy3
Label protocol Labelling was performed using the miRCURY LNA™ microRNA Power Labeling Kit, according to the manufacturer protocol http://www.exiqon.com/ls/Documents/Scientific/miRCURY-LNA%E2%84%A2-microRNA-Array-v11-other-species-manual.pdf)
 
Channel 2
Source name adipose tissue from standard fat diet mouse
Organism Mus musculus
Characteristics tissue: Adipose tissue
Extracted molecule total RNA
Extraction protocol Total RNA extracted using guanidinium thiocyanate following manufacturer's instructions
Label Hy5
Label protocol Labelling was performed using the miRCURY LNA™ microRNA Power Labeling Kit, according to the manufacturer protocol http://www.exiqon.com/ls/Documents/Scientific/miRCURY-LNA%E2%84%A2-microRNA-Array-v11-other-species-manual.pdf)
 
 
Hybridization protocol Hybridization was performed according to the manufacturer protocol (http://www.exiqon.com/ls/Documents/Scientific/miRCURY-LNA%E2%84%A2-microRNA-Array-v11-other-species-manual.pdf)
Scan protocol The miRCURY™ LNA array microarray slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA)
Description Technical replicate 2 of 2.
Data processing Image analysis was carried out using the ImaGene 8.0 software (BioDiscovery, Inc., USA). LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal.
 
Submission date Sep 27, 2011
Last update date Nov 30, 2011
Contact name Apostolos Zaravinos
E-mail(s) zaravinos.apostolos@ucy.ac.cy
Organization name University of Cyprus
Department Department of Biological Sciences
Lab Molecular Medicine Research Center (MMRC)
Street address Kallipoleos 75
City Nicosia
State/province Nicosia
ZIP/Postal code 1678
Country Cyprus
 
Platform ID GPL7723
Series (1)
GSE32419 MicroRNA expression differences in adipose tissue between high and low fat diet mice

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Hy3/Hy5) representing (high fat diet mouse/standard diet mouse)

Data table
ID_REF VALUE
17748 0.242741264
17888 0.256003325
29502 0.392952969
42769 0.263906865
19004 0.25312651
42668 0.24622741
10914 0.190457234
17750 0.1456692
42505 0.198205734
42459 -0.14785444
17752 0.150039574
33401 -0.02287662
42768 0.14630792
19602 0.325930167
42463 -0.122912417
42778
9938 -0.297278371
19580 -0.297989314
42534 -0.440812621
42573 1.203087346

Total number of rows: 608

Table truncated, full table size 10 Kbytes.




Supplementary file Size Download File type/resource
GSM802368.txt.gz 20.3 Kb (ftp)(http) TXT
Processed data included within Sample table

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