|
Status |
Public on Nov 30, 2011 |
Title |
High fat diet mouse2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
adipose tissue from high fat diet mouse
|
Organism |
Mus musculus |
Characteristics |
tissue: Adipose tissue
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using guanidinium thiocyanate following manufacturer's instructions
|
Label |
Hy3
|
Label protocol |
Labelling was performed using the miRCURY LNA™ microRNA Power Labeling Kit, according to the manufacturer protocol http://www.exiqon.com/ls/Documents/Scientific/miRCURY-LNA%E2%84%A2-microRNA-Array-v11-other-species-manual.pdf)
|
|
|
Channel 2 |
Source name |
adipose tissue from standard fat diet mouse
|
Organism |
Mus musculus |
Characteristics |
tissue: Adipose tissue
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using guanidinium thiocyanate following manufacturer's instructions
|
Label |
Hy5
|
Label protocol |
Labelling was performed using the miRCURY LNA™ microRNA Power Labeling Kit, according to the manufacturer protocol http://www.exiqon.com/ls/Documents/Scientific/miRCURY-LNA%E2%84%A2-microRNA-Array-v11-other-species-manual.pdf)
|
|
|
|
Hybridization protocol |
Hybridization was performed according to the manufacturer protocol (http://www.exiqon.com/ls/Documents/Scientific/miRCURY-LNA%E2%84%A2-microRNA-Array-v11-other-species-manual.pdf)
|
Scan protocol |
The miRCURY™ LNA array microarray slides were scanned using the Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA)
|
Description |
Technical replicate 2 of 2.
|
Data processing |
Image analysis was carried out using the ImaGene 8.0 software (BioDiscovery, Inc., USA). LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal.
|
|
|
Submission date |
Sep 27, 2011 |
Last update date |
Nov 30, 2011 |
Contact name |
Apostolos Zaravinos |
E-mail(s) |
zaravinos.apostolos@ucy.ac.cy
|
Organization name |
University of Cyprus
|
Department |
Department of Biological Sciences
|
Lab |
Molecular Medicine Research Center (MMRC)
|
Street address |
Kallipoleos 75
|
City |
Nicosia |
State/province |
Nicosia |
ZIP/Postal code |
1678 |
Country |
Cyprus |
|
|
Platform ID |
GPL7723 |
Series (1) |
GSE32419 |
MicroRNA expression differences in adipose tissue between high and low fat diet mice |
|