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Sample GSM801869 Query DataSets for GSM801869
Status Public on Nov 30, 2011
Title L87/4 human stem cells
Sample type RNA
 
Source name L87/4 human stem cells
Organism Homo sapiens
Characteristics cell type: possible osteosarcoma progenitor
cell line: L87/4 human stem cells
Growth protocol grown in RPMI 1640 (Invitrogen, Karlsruhe, Germany) supplemented with 2% L-glutamine (Invitrogen) and 10% fetal calf serum (FCS)
Extracted molecule total RNA
Extraction protocol using the mirVana™ miRNA Isolation Kit (Ambion, Austin, USA) according to the manufacturer´s instructions
Label biotin
Label protocol MessageAmp (1. round) and MessageAmp II-Biotin Enhanced (2.round) 2-cycle protocoll (Ambion)
 
Hybridization protocol According to the Affymetrix expression protocol
Scan protocol According to the Affymetrix expression protocol
Description Transcriptom determination for correlation with miRNA expression
Data processing RMA probeset summary and normalisation employing the loess smoother
 
Submission date Sep 26, 2011
Last update date Nov 30, 2011
Contact name Jan Smida
E-mail(s) smida@helmholtz-muenchen.de
Phone 00498937813797
Fax 00498931873381
Organization name HelmholtzZentrum
Department CCg Osteosarcoma
Lab 57/129
Street address Ingolstaedter Land 1
City Neuherberg
ZIP/Postal code D-85764
Country Germany
 
Platform ID GPL6244
Series (1)
GSE32395 miRNA profiling in osteosarcoma: a cell line based approach with correlation to gene expression

Data table header descriptions
ID_REF
VALUE RMA signal intensity (linear)

Data table
ID_REF VALUE
7896736 371
7896740 270
7896742 685
7896744 405
7896746 792
7896748 1108
7896752 1639
7896754 200
7896761 128
7896779 123
7896798 186
7896817 138
7896822 380
7896865 154
7896908 239
7896921 126
7896929 152
7896952 418
7896961 344
7896985 108

Total number of rows: 26890

Table truncated, full table size 305 Kbytes.




Supplementary file Size Download File type/resource
GSM801869_L_87.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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