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Sample GSM801868 Query DataSets for GSM801868
Status Public on Nov 30, 2011
Title MG-63 osteosarcoma cell line CRL-1427
Sample type RNA
 
Source name MG-63 osteosarcoma cell line CRL-1427
Organism Homo sapiens
Characteristics cell type: osteosarcoma derivate
cell line: MG-63 osteosarcoma cell line CRL-1427
Growth protocol grown in RPMI 1640 (Invitrogen, Karlsruhe, Germany) supplemented with 2% L-glutamine (Invitrogen) and 10% fetal calf serum (FCS)
Extracted molecule total RNA
Extraction protocol using the mirVana™ miRNA Isolation Kit (Ambion, Austin, USA) according to the manufacturer´s instructions
Label biotin
Label protocol MessageAmp (1. round) and MessageAmp II-Biotin Enhanced (2.round) 2-cycle protocoll (Ambion)
 
Hybridization protocol According to the Affymetrix expression protocol
Scan protocol According to the Affymetrix expression protocol
Description Transcriptom determination for correlation with miRNA expression
Data processing RMA probeset summary and normalisation employing the loess smoother
 
Submission date Sep 26, 2011
Last update date Nov 30, 2011
Contact name Jan Smida
E-mail(s) smida@helmholtz-muenchen.de
Phone 00498937813797
Fax 00498931873381
Organization name HelmholtzZentrum
Department CCg Osteosarcoma
Lab 57/129
Street address Ingolstaedter Land 1
City Neuherberg
ZIP/Postal code D-85764
Country Germany
 
Platform ID GPL6244
Series (1)
GSE32395 miRNA profiling in osteosarcoma: a cell line based approach with correlation to gene expression

Data table header descriptions
ID_REF
VALUE RMA signal intensity (linear)

Data table
ID_REF VALUE
7896736 172
7896740 116
7896742 234
7896744 145
7896746 560
7896748 861
7896752 1569
7896754 195
7896761 135
7896779 139
7896798 183
7896817 261
7896822 476
7896865 162
7896908 298
7896921 134
7896929 193
7896952 405
7896961 312
7896985 168

Total number of rows: 26890

Table truncated, full table size 305 Kbytes.




Supplementary file Size Download File type/resource
GSM801868_MG_63.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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