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Sample GSM801867 Query DataSets for GSM801867
Status Public on Nov 30, 2011
Title SJSA osteosarcoma cell line CRL-2098
Sample type RNA
 
Source name SJSA osteosarcoma cell line CRL-2098
Organism Homo sapiens
Characteristics cell type: osteosarcoma derivate
cell line: SJSA osteosarcoma cell line CRL-2098
Growth protocol grown in RPMI 1640 (Invitrogen, Karlsruhe, Germany) supplemented with 2% L-glutamine (Invitrogen) and 10% fetal calf serum (FCS)
Extracted molecule total RNA
Extraction protocol using the mirVana™ miRNA Isolation Kit (Ambion, Austin, USA) according to the manufacturer´s instructions
Label biotin
Label protocol MessageAmp (1. round) and MessageAmp II-Biotin Enhanced (2.round) 2-cycle protocoll (Ambion)
 
Hybridization protocol According to the Affymetrix expression protocol
Scan protocol According to the Affymetrix expression protocol
Description Transcriptom determination for correlation with miRNA expression
Data processing RMA probeset summary and normalisation employing the loess smoother
 
Submission date Sep 26, 2011
Last update date Nov 30, 2011
Contact name Jan Smida
E-mail(s) smida@helmholtz-muenchen.de
Phone 00498937813797
Fax 00498931873381
Organization name HelmholtzZentrum
Department CCg Osteosarcoma
Lab 57/129
Street address Ingolstaedter Land 1
City Neuherberg
ZIP/Postal code D-85764
Country Germany
 
Platform ID GPL6244
Series (1)
GSE32395 miRNA profiling in osteosarcoma: a cell line based approach with correlation to gene expression

Data table header descriptions
ID_REF
VALUE RMA signal intensity (linear)

Data table
ID_REF VALUE
7896736 178
7896740 111
7896742 312
7896744 123
7896746 750
7896748 1020
7896752 2805
7896754 151
7896761 116
7896779 124
7896798 154
7896817 142
7896822 332
7896865 122
7896908 283
7896921 126
7896929 158
7896952 410
7896961 342
7896985 119

Total number of rows: 26890

Table truncated, full table size 304 Kbytes.




Supplementary file Size Download File type/resource
GSM801867_SJSA_01.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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