|
Status |
Public on Nov 30, 2011 |
Title |
MNNG/HOS osteosarcoma cell line CRL-1547 |
Sample type |
RNA |
|
|
Source name |
MNNG/HOS osteosarcoma cell line CRL-1547
|
Organism |
Homo sapiens |
Characteristics |
cell type: osteosarcoma derivate cell line: MNNG/HOS osteosarcoma cell line CRL-1547
|
Growth protocol |
grown in RPMI 1640 (Invitrogen, Karlsruhe, Germany) supplemented with 2% L-glutamine (Invitrogen) and 10% fetal calf serum (FCS)
|
Extracted molecule |
total RNA |
Extraction protocol |
using the mirVana™ miRNA Isolation Kit (Ambion, Austin, USA) according to the manufacturer´s instructions
|
Label |
biotin
|
Label protocol |
MessageAmp (1. round) and MessageAmp II-Biotin Enhanced (2.round) 2-cycle protocoll (Ambion)
|
|
|
Hybridization protocol |
According to the Affymetrix expression protocol
|
Scan protocol |
According to the Affymetrix expression protocol
|
Description |
Transcriptom determination for correlation with miRNA expression
|
Data processing |
RMA probeset summary and normalisation employing the loess smoother
|
|
|
Submission date |
Sep 26, 2011 |
Last update date |
Nov 30, 2011 |
Contact name |
Jan Smida |
E-mail(s) |
smida@helmholtz-muenchen.de
|
Phone |
00498937813797
|
Fax |
00498931873381
|
Organization name |
HelmholtzZentrum
|
Department |
CCg Osteosarcoma
|
Lab |
57/129
|
Street address |
Ingolstaedter Land 1
|
City |
Neuherberg |
ZIP/Postal code |
D-85764 |
Country |
Germany |
|
|
Platform ID |
GPL6244 |
Series (1) |
GSE32395 |
miRNA profiling in osteosarcoma: a cell line based approach with correlation to gene expression |
|