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Sample GSM801866 Query DataSets for GSM801866
Status Public on Nov 30, 2011
Title MNNG/HOS osteosarcoma cell line CRL-1547
Sample type RNA
 
Source name MNNG/HOS osteosarcoma cell line CRL-1547
Organism Homo sapiens
Characteristics cell type: osteosarcoma derivate
cell line: MNNG/HOS osteosarcoma cell line CRL-1547
Growth protocol grown in RPMI 1640 (Invitrogen, Karlsruhe, Germany) supplemented with 2% L-glutamine (Invitrogen) and 10% fetal calf serum (FCS)
Extracted molecule total RNA
Extraction protocol using the mirVana™ miRNA Isolation Kit (Ambion, Austin, USA) according to the manufacturer´s instructions
Label biotin
Label protocol MessageAmp (1. round) and MessageAmp II-Biotin Enhanced (2.round) 2-cycle protocoll (Ambion)
 
Hybridization protocol According to the Affymetrix expression protocol
Scan protocol According to the Affymetrix expression protocol
Description Transcriptom determination for correlation with miRNA expression
Data processing RMA probeset summary and normalisation employing the loess smoother
 
Submission date Sep 26, 2011
Last update date Nov 30, 2011
Contact name Jan Smida
E-mail(s) smida@helmholtz-muenchen.de
Phone 00498937813797
Fax 00498931873381
Organization name HelmholtzZentrum
Department CCg Osteosarcoma
Lab 57/129
Street address Ingolstaedter Land 1
City Neuherberg
ZIP/Postal code D-85764
Country Germany
 
Platform ID GPL6244
Series (1)
GSE32395 miRNA profiling in osteosarcoma: a cell line based approach with correlation to gene expression

Data table header descriptions
ID_REF
VALUE RMA signal intensity (linear)

Data table
ID_REF VALUE
7896736 290
7896740 207
7896742 366
7896744 199
7896746 746
7896748 808
7896752 2044
7896754 121
7896761 133
7896779 149
7896798 186
7896817 161
7896822 395
7896865 150
7896908 343
7896921 144
7896929 175
7896952 566
7896961 570
7896985 138

Total number of rows: 26890

Table truncated, full table size 305 Kbytes.




Supplementary file Size Download File type/resource
GSM801866_MNNG.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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