|
| Status |
Public on Nov 30, 2011 |
| Title |
U2-OS osteosarcoma cell line HTB-96 |
| Sample type |
RNA |
| |
|
| Source name |
U2-OS osteosarcoma cell line HTB-96
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: osteosarcoma derivate
cell line: U2-OS osteosarcoma cell line HTB-96
|
| Growth protocol |
grown in RPMI 1640 (Invitrogen, Karlsruhe, Germany) supplemented with 2% L-glutamine (Invitrogen) and 10% fetal calf serum (FCS)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
using the mirVana™ miRNA Isolation Kit (Ambion, Austin, USA) according to the manufacturer´s instructions
|
| Label |
biotin
|
| Label protocol |
MessageAmp (1. round) and MessageAmp II-Biotin Enhanced (2.round) 2-cycle protocoll (Ambion)
|
| |
|
| Hybridization protocol |
According to the Affymetrix expression protocol
|
| Scan protocol |
According to the Affymetrix expression protocol
|
| Description |
Transcriptom determination for correlation with miRNA expression
|
| Data processing |
RMA probeset summary and normalisation employing the loess smoother
|
| |
|
| Submission date |
Sep 26, 2011 |
| Last update date |
Nov 30, 2011 |
| Contact name |
Jan Smida |
| E-mail(s) |
smida@helmholtz-muenchen.de
|
| Phone |
00498937813797
|
| Fax |
00498931873381
|
| Organization name |
HelmholtzZentrum
|
| Department |
CCg Osteosarcoma
|
| Lab |
57/129
|
| Street address |
Ingolstaedter Land 1
|
| City |
Neuherberg |
| ZIP/Postal code |
D-85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE32395 |
miRNA profiling in osteosarcoma: a cell line based approach with correlation to gene expression |
|
