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Sample GSM801863 Query DataSets for GSM801863
Status Public on Nov 30, 2011
Title HOS-58 osteosarcoma cell line
Sample type RNA
 
Source name HOS-58 osteosarcoma cell line
Organism Homo sapiens
Characteristics cell type: osteosarcoma derivate
cell line: HOS-58 osteosarcoma cell line
Growth protocol grown in RPMI 1640 (Invitrogen, Karlsruhe, Germany) supplemented with 2% L-glutamine (Invitrogen) and 10% fetal calf serum (FCS)
Extracted molecule total RNA
Extraction protocol using the mirVana™ miRNA Isolation Kit (Ambion, Austin, USA) according to the manufacturer´s instructions
Label biotin
Label protocol MessageAmp (1. round) and MessageAmp II-Biotin Enhanced (2.round) 2-cycle protocoll (Ambion)
 
Hybridization protocol According to the Affymetrix expression protocol
Scan protocol According to the Affymetrix expression protocol
Description Transcriptom determination for correlation with miRNA expression
Data processing RMA probeset summary and normalisation employing the loess smoother
 
Submission date Sep 26, 2011
Last update date Nov 30, 2011
Contact name Jan Smida
E-mail(s) smida@helmholtz-muenchen.de
Phone 00498937813797
Fax 00498931873381
Organization name HelmholtzZentrum
Department CCg Osteosarcoma
Lab 57/129
Street address Ingolstaedter Land 1
City Neuherberg
ZIP/Postal code D-85764
Country Germany
 
Platform ID GPL6244
Series (1)
GSE32395 miRNA profiling in osteosarcoma: a cell line based approach with correlation to gene expression

Data table header descriptions
ID_REF
VALUE RMA signal intensity (linear)

Data table
ID_REF VALUE
7896736 350
7896740 321
7896742 819
7896744 404
7896746 1051
7896748 964
7896752 2500
7896754 286
7896761 140
7896779 113
7896798 175
7896817 129
7896822 145
7896865 167
7896908 208
7896921 162
7896929 191
7896952 356
7896961 252
7896985 127

Total number of rows: 26890

Table truncated, full table size 305 Kbytes.




Supplementary file Size Download File type/resource
GSM801863_HOS_58.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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