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Sample GSM801862 Query DataSets for GSM801862
Status Public on Nov 30, 2011
Title HOS osteosarcoma cell line CRL-1543
Sample type RNA
 
Source name HOS osteosarcoma cell line CRL-1543
Organism Homo sapiens
Characteristics cell type: osteosarcoma derivate
cell line: HOS osteosarcoma cell line CRL-1543
Growth protocol grown in RPMI 1640 (Invitrogen, Karlsruhe, Germany) supplemented with 2% L-glutamine (Invitrogen) and 10% fetal calf serum (FCS)
Extracted molecule total RNA
Extraction protocol using the mirVana™ miRNA Isolation Kit (Ambion, Austin, USA) according to the manufacturer´s instructions
Label biotin
Label protocol MessageAmp (1. round) and MessageAmp II-Biotin Enhanced (2.round) 2-cycle protocoll (Ambion)
 
Hybridization protocol According to the Affymetrix expression protocol
Scan protocol According to the Affymetrix expression protocol
Description Transcriptom determination for correlation with miRNA expression
Data processing RMA probeset summary and normalisation employing the loess smoother
 
Submission date Sep 26, 2011
Last update date Nov 30, 2011
Contact name Jan Smida
E-mail(s) smida@helmholtz-muenchen.de
Phone 00498937813797
Fax 00498931873381
Organization name HelmholtzZentrum
Department CCg Osteosarcoma
Lab 57/129
Street address Ingolstaedter Land 1
City Neuherberg
ZIP/Postal code D-85764
Country Germany
 
Platform ID GPL6244
Series (1)
GSE32395 miRNA profiling in osteosarcoma: a cell line based approach with correlation to gene expression

Data table header descriptions
ID_REF
VALUE RMA signal intensity (linear)

Data table
ID_REF VALUE
7896736 374
7896740 311
7896742 713
7896744 363
7896746 764
7896748 818
7896752 2104
7896754 243
7896761 148
7896779 117
7896798 167
7896817 115
7896822 145
7896865 158
7896908 225
7896921 150
7896929 173
7896952 336
7896961 314
7896985 138

Total number of rows: 26890

Table truncated, full table size 305 Kbytes.




Supplementary file Size Download File type/resource
GSM801862_HOS.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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