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Sample GSM801629 Query DataSets for GSM801629
Status Public on May 01, 2012
Title Parathyroid - Control 24 h - Replicate 1
Sample type RNA
 
Source name Parathyroid_Control_24h
Organism Homo sapiens
Characteristics tissue: Parthyroid Adenoma
Treatment protocol Expression profiling was done in parathyroid adenomas subjected to prolactin treatment in culture. In addition, corresponding paraffin sections were obtained for verification of PRLr expression by immunohistochemistry. 200 mg/L prolactin (recombinant human prolactin, Cat. No. JM-4687-50, MBL Woburn, MA) was added to 1× 10^6 attached parathyroid tumour cells. Cells were harvested using RNAlater (QIAGEN) and homogenized with QIAshredder for RNA extraction after 3 h and 24 h in culture, respectively. Negative controls were collected in parallel with each case at the same time points. RNA was extracted using QIA Cube, and quality assessed with Bioanalyser and Nanodrop.
Extracted molecule total RNA
Extraction protocol Parathyroid adenoma samples for culturing and functional studies were obtained at surgery. Up to one third of the tumour was collected from each sample, while the majority of the tissue was sent to routine histopathological diagnostics. Fresh tissue samples were quickly transferred in cold culturing media to the nearby laboratory, and then isolated and cultured using previously described methods. All experiments were performed within 72 hours after isolation.
Label biotin
Label protocol Total RNA was converted to sense strand cDNA using Ambion WT Expression kit. For fragmentation and labeling WT GeneChip WT target Labeling kit was used.
 
Hybridization protocol Hybridization to Affymetrix Human Gene 1.1 ST Array plates was performed using GeneTitan Hybridization, Wash, and Stain Kit for WT Array Plates.
Scan protocol Array plates were scanned using Affymetrix GeneChip HT Array Plate Scanner according to standard protocols (Affymetrix Inc., Santa Clara, CA).
Description Gene expression of parathyroid adenoma
Data processing Preprocessing was performed in Affymetrix Expression Console (v. 1.1) using the following methods: Summarization: PLIER, Background Correction: PM-GCBG, Normalization: Global Median.
 
Submission date Sep 26, 2011
Last update date May 01, 2012
Contact name Felix Haglund
Organization name Karolinska Institutet
Department MMK
Lab C Larsson
Street address Karolinska Universitetssjukhuset Solna, CMM L8:01
City Stockholm
ZIP/Postal code 17176
Country Sweden
 
Platform ID GPL11532
Series (1)
GSE32387 Prolactin stimulation of parathyroid adenomas

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
7892501 20.0502
7892502 30.16
7892503 12.1971
7892504 244.034
7892505 4.94216
7892506 11.113
7892507 20.9959
7892508 79.0371
7892509 2138.34
7892510 22.8103
7892511 22.6249
7892512 0.861609
7892513 7.20515
7892514 1118.6
7892515 874.721
7892516 1.94945
7892517 72.5496
7892518 2.73819
7892519 11.3246
7892520 267.382

Total number of rows: 33297

Table truncated, full table size 517 Kbytes.




Supplementary file Size Download File type/resource
GSM801629_FH03_C05.CEL.gz 4.5 Mb (ftp)(http) CEL
GSM801629_FH03_C05.plier-gene-default.chp.gz 263.6 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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