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Sample GSM801628 Query DataSets for GSM801628
Status Public on May 01, 2012
Title Parathyroid - Prolactin 3 h - Replicate 1
Sample type RNA
 
Source name Parathyroid_200 mg/L Prolactin_3h
Organism Homo sapiens
Characteristics tissue: Parthyroid Adenoma
Treatment protocol Expression profiling was done in parathyroid adenomas subjected to prolactin treatment in culture. In addition, corresponding paraffin sections were obtained for verification of PRLr expression by immunohistochemistry. 200 mg/L prolactin (recombinant human prolactin, Cat. No. JM-4687-50, MBL Woburn, MA) was added to 1× 10^6 attached parathyroid tumour cells. Cells were harvested using RNAlater (QIAGEN) and homogenized with QIAshredder for RNA extraction after 3 h and 24 h in culture, respectively. Negative controls were collected in parallel with each case at the same time points. RNA was extracted using QIA Cube, and quality assessed with Bioanalyser and Nanodrop.
Extracted molecule total RNA
Extraction protocol Parathyroid adenoma samples for culturing and functional studies were obtained at surgery. Up to one third of the tumour was collected from each sample, while the majority of the tissue was sent to routine histopathological diagnostics. Fresh tissue samples were quickly transferred in cold culturing media to the nearby laboratory, and then isolated and cultured using previously described methods. All experiments were performed within 72 hours after isolation.
Label biotin
Label protocol Total RNA was converted to sense strand cDNA using Ambion WT Expression kit. For fragmentation and labeling WT GeneChip WT target Labeling kit was used.
 
Hybridization protocol Hybridization to Affymetrix Human Gene 1.1 ST Array plates was performed using GeneTitan Hybridization, Wash, and Stain Kit for WT Array Plates.
Scan protocol Array plates were scanned using Affymetrix GeneChip HT Array Plate Scanner according to standard protocols (Affymetrix Inc., Santa Clara, CA).
Description Gene expression of parathyroid adenoma
Data processing Preprocessing was performed in Affymetrix Expression Console (v. 1.1) using the following methods: Summarization: PLIER, Background Correction: PM-GCBG, Normalization: Global Median.
 
Submission date Sep 26, 2011
Last update date May 01, 2012
Contact name Felix Haglund
Organization name Karolinska Institutet
Department MMK
Lab C Larsson
Street address Karolinska Universitetssjukhuset Solna, CMM L8:01
City Stockholm
ZIP/Postal code 17176
Country Sweden
 
Platform ID GPL11532
Series (1)
GSE32387 Prolactin stimulation of parathyroid adenomas

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
7892501 31.957
7892502 26.5249
7892503 23.1569
7892504 220.423
7892505 2.63189
7892506 11.6007
7892507 17.2916
7892508 40.9508
7892509 1667.36
7892510 14.1369
7892511 16.4183
7892512 5.66752
7892513 6.6749
7892514 1074.56
7892515 1123.29
7892516 5.59809
7892517 33.8374
7892518 8.00182
7892519 12.4346
7892520 240.623

Total number of rows: 33297

Table truncated, full table size 517 Kbytes.




Supplementary file Size Download File type/resource
GSM801628_FH02_B05.CEL.gz 4.3 Mb (ftp)(http) CEL
GSM801628_FH02_B05.plier-gene-default.chp.gz 263.6 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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