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Sample GSM801199 Query DataSets for GSM801199
Status Public on Nov 08, 2011
Title Subject 1_t=30days_placebo
Sample type RNA
Source name after 30 days intervention with placebo
Organism Homo sapiens
Characteristics ethnic background: Caucasian
gender: male
age: 45-65 years
tissue: vastus lateralis muscle
Treatment protocol The design of the study was a randomized cross-over design with two different treatments: placebo and resVida (150mg/day trans-resveratrol). Subjects were instructed to take the first supplement on the day after baseline measurements (d1) and the last supplement in the evening on day 29. In the morning of day 30, after a 36h in the respiration chamber, a muscle biopsy was taken, which was followed by the withdrawal of a fasting blood sample.
Growth protocol Ten healthy obese men (age 45–65 years; BMI 30-35 kg/m2) participated in a double-blind randomized cross-over study. Exclusion criteria were unstable body weight (weight gain or loss > 3 kg in the last three months), total body fat (%) < 25%, fasting plasma glucose > 6.1 mmol/l, hemoglobin < 7.8 mmol/l, engagement in programmed exercise > 2 hours total per week, impaired kidney and/ or hepatic function, first- or second-degree family member with type 2 diabetes mellitus, any medical condition requiring treatment and/or medication use, intake of dietary supplements except vitamins and minerals, unwilling to restrict high-resveratrol-containing foods, current alcohol consumption > 20 grams/day, participation in another biomedical study within 1 month before the first screening visit, a contraindication to MRI scanning. All subjects were informed about the nature of the study and written informed consent was obtained before study participation. The local Medical Ethical Committee of Maastricht University Medical Centre approved the study protocol.
Extracted molecule total RNA
Extraction protocol Skeletal muscle biopsies were taken in the morning of day 30 after an overnight fast. Biopsies were obtained from the vastus lateralis muscle under local anesthesia of the skin and fascia using the Bergström method with suction, cleaned from any visible fat and blood, immediately frozen in isopentane at its melting point, and stored at −80C until analysis.
Label biotin
Label protocol The Affymetrix GeneChip WT Sense Target Labeling and Control Reagents kit (P/N 900652) was used for the preparation of labelled cDNA from 100ng of total RNA without rRNA reduction. A detailed description can be found in the User Manual, Chapter 3 (P/N 701880, revision 5).
Hybridization protocol The Affymetrix GeneChip Human Gene 1.1 ST 24-array plate consists of 24 single Gene 1.1 ST arrays arranged into the standard 96 well plate format. For this experiment 20 arrays were used. Array hybridization, washing and scanning were performed on a GeneTitan Instrument according to the manufacturer’s recommendations.
Scan protocol Arrays were scanned on an Affymetrix GeneTitan instrument.
Description G032A_A07_2_JHER1_p2.CEL
Data processing Expression estimates were calculated applying the RMA algorithm in the Bioconductor library 'Oligo' (v1.16.0).
Submission date Sep 23, 2011
Last update date Nov 08, 2011
Contact name Guido Hooiveld
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
Platform ID GPL11532
Series (1)
GSE32357 Calorie restriction-like effects of 30 days of resveratrol supplementation on energy metabolism and metabolic profile in obese humans

Data table header descriptions
VALUE log2 RMA signal

Data table
7892501 2.463439
7892502 3.348783
7892503 2.162132
7892504 7.352416
7892505 2.070977
7892506 2.381301
7892507 3.021903
7892508 2.538404
7892509 8.914657
7892510 2.953608
7892511 2.647855
7892512 2.537534
7892513 1.6075
7892514 7.266882
7892515 7.143421
7892516 1.275163
7892517 4.66659
7892518 1.728446
7892519 1.649207
7892520 6.743622

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.

Supplementary file Size Download File type/resource
GSM801199_G032A_A07_2_JHER1_P2.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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