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Sample GSM800843 Query DataSets for GSM800843
Status Public on Nov 30, 2011
Title pre-B_12h_b-Est_rep1
Sample type RNA
Source name B to M transdifferentiation sample
Organism Mus musculus
Characteristics cell type: Bone marrow derived cells (pre-B)
infection: C/EBPaER-GFP retrovirus
agent: b-Est
time: 12h
Treatment protocol GFP pos. cells were sorted and afetr re-seeding on stroma induced with b-Est. GFP pos cell were sorted at different imes thereafter
Growth protocol Pre-B cells were isolated, placed in duplicate on stromal cells plus cytokines, infected with an C/EBPaER-GFP retrovirus, GFP pos. cells sorted and after re-seeding on stroma induced with b-Est. Green cells were sorted at different times
Extracted molecule total RNA
Extraction protocol RNA was isolated by Trizol
Label Biotin
Label protocol RNAs were processed for Affymetrix 430.2 arrays as described in the manufacturer's instructions
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description Gene expression data from B to M transdifferentiation
Data processing The data have been normalized with RMA method, using Bioconductor in R
Submission date Sep 23, 2011
Last update date Nov 30, 2011
Contact name Thien-Phong Vu Manh
Organization name CIML
Street address 163 avenue de Luminy
City marseille
ZIP/Postal code 13288
Country France
Platform ID GPL1261
Series (1)
GSE32330 Expression data from C/EBP alpha induced transdifferentiation of pre-B cells into macrophages

Data table header descriptions
VALUE log2 normalized signal

Data table
1415670_at 8.59155604618917
1415671_at 9.25686843912469
1415672_at 9.43471259808168
1415673_at 7.71100655280396
1415674_a_at 8.28139963203865
1415675_at 8.96548523665816
1415676_a_at 11.0324025102682
1415677_at 6.85489066829912
1415678_at 9.95805834787025
1415679_at 10.8518262541738
1415680_at 10.047782693069
1415681_at 8.73232043297197
1415682_at 6.10378708518007
1415683_at 11.7031453425920
1415684_at 6.49326230496579
1415685_at 7.4992655695086
1415686_at 8.97937267869303
1415687_a_at 10.6180631528018
1415688_at 9.56651679147233
1415689_s_at 7.66665439566333

Total number of rows: 45101

Table truncated, full table size 1246 Kbytes.

Supplementary file Size Download File type/resource
GSM800843.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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