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Sample GSM800842 Query DataSets for GSM800842
Status Public on Nov 30, 2011
Title pre-B_3h_b-Est_rep2
Sample type RNA
Source name B to M transdifferentiation sample
Organism Mus musculus
Characteristics cell type: Bone marrow derived cells (pre-B)
infection: C/EBPaER-GFP retrovirus
agent: b-Est
time: 3h
Treatment protocol GFP pos. cells were sorted and afetr re-seeding on stroma induced with b-Est. GFP pos cell were sorted at different imes thereafter
Growth protocol Pre-B cells were isolated, placed in duplicate on stromal cells plus cytokines, infected with an C/EBPaER-GFP retrovirus, GFP pos. cells sorted and after re-seeding on stroma induced with b-Est. Green cells were sorted at different times
Extracted molecule total RNA
Extraction protocol RNA was isolated by Trizol
Label Biotin
Label protocol RNAs were processed for Affymetrix 430.2 arrays as described in the manufacturer's instructions
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description Gene expression data from B to M transdifferentiation
Data processing The data have been normalized with RMA method, using Bioconductor in R
Submission date Sep 23, 2011
Last update date Nov 30, 2011
Contact name Thien-Phong Vu Manh
Organization name CIML
Street address 163 avenue de Luminy
City marseille
ZIP/Postal code 13288
Country France
Platform ID GPL1261
Series (1)
GSE32330 Expression data from C/EBP alpha induced transdifferentiation of pre-B cells into macrophages

Data table header descriptions
VALUE log2 normalized signal

Data table
1415670_at 9.22092251562716
1415671_at 9.04430742200952
1415672_at 10.4795107207332
1415673_at 9.4551214752947
1415674_a_at 8.77300649589875
1415675_at 8.9325945328208
1415676_a_at 11.9992419126942
1415677_at 7.46399730413695
1415678_at 10.2967058200889
1415679_at 10.9681100672080
1415680_at 10.3256653207972
1415681_at 9.35402870395532
1415682_at 7.10273170024275
1415683_at 12.1812117654046
1415684_at 6.77202697735116
1415685_at 7.59919122443612
1415686_at 9.10624944013545
1415687_a_at 10.0131267087973
1415688_at 10.2010823240638
1415689_s_at 9.00870646476578

Total number of rows: 45101

Table truncated, full table size 1246 Kbytes.

Supplementary file Size Download File type/resource
GSM800842.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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