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Sample GSM800840 Query DataSets for GSM800840
Status Public on Nov 30, 2011
Title pre-B_3h_EtOH_rep2
Sample type RNA
 
Source name B to M transdifferentiation sample
Organism Mus musculus
Characteristics cell type: Bone marrow derived cells (pre-B)
infection: C/EBPaER-GFP retrovirus
agent: EtOH
time: 3h
Treatment protocol GFP pos. cells were sorted and afetr re-seeding on stroma induced with b-Est. GFP pos cell were sorted at different imes thereafter
Growth protocol Pre-B cells were isolated, placed in duplicate on stromal cells plus cytokines, infected with an C/EBPaER-GFP retrovirus, GFP pos. cells sorted and after re-seeding on stroma induced with b-Est. Green cells were sorted at different times
Extracted molecule total RNA
Extraction protocol RNA was isolated by Trizol
Label Biotin
Label protocol RNAs were processed for Affymetrix 430.2 arrays as described in the manufacturer's instructions
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description Gene expression data from B to M transdifferentiation
2390_aER_3h_EtOH_2.CEL
Data processing The data have been normalized with RMA method, using Bioconductor in R
 
Submission date Sep 23, 2011
Last update date Nov 30, 2011
Contact name Thien-Phong Vu Manh
Organization name CIML
Street address 163 avenue de Luminy
City marseille
ZIP/Postal code 13288
Country France
 
Platform ID GPL1261
Series (1)
GSE32330 Expression data from C/EBP alpha induced transdifferentiation of pre-B cells into macrophages

Data table header descriptions
ID_REF
VALUE log2 normalized signal

Data table
ID_REF VALUE
1415670_at 9.15301076922341
1415671_at 8.87435208403968
1415672_at 10.5331143793923
1415673_at 9.70002561220483
1415674_a_at 8.97845075391897
1415675_at 8.7375502083345
1415676_a_at 12.0578374567653
1415677_at 6.80760923960155
1415678_at 10.1711114164317
1415679_at 10.9452718609087
1415680_at 10.3274779333526
1415681_at 9.29055624786569
1415682_at 7.07103373758869
1415683_at 12.0284876534211
1415684_at 7.04972122422489
1415685_at 8.07843557494519
1415686_at 8.94867243707512
1415687_a_at 10.1259088605866
1415688_at 10.3577940373978
1415689_s_at 9.10023918053592

Total number of rows: 45101

Table truncated, full table size 1246 Kbytes.




Supplementary file Size Download File type/resource
GSM800840.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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