|
Status |
Public on Jun 10, 2012 |
Title |
HCT116_DKO_WGA_rep7_glioblstoma study |
Sample type |
genomic |
|
|
Source name |
Genomic DNA from HCT116 DNMT1;DNMT3 double knockout cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: HCT116 DNMT1;DNMT3 double knockout idh1/2 genotype: wildtype
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted using the QIAamp DNA mini kit, according to the manufacturer's protocol
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina protocol
|
|
|
Hybridization protocol |
Genomic DNA was bisulfite converted with the Zymo Research EZ DNA Methylation kit. Bisulfite converted DNA was amplified, fragmented and hybridized to Illumina Human Methylation450 Beadchip using standard Illumina protocol
|
Scan protocol |
BeadChips were imaged with the Illumina HiScan scanner using standard settings
|
Data processing |
Mean background-corrected and normalized signal intensities of the methylation (M) and unmethylated (U) estimates for each CpG locus were extracted using Illumina BeadStudio software
|
|
|
Submission date |
Sep 21, 2011 |
Last update date |
Jun 10, 2012 |
Contact name |
Derek Chiang |
E-mail(s) |
chiang@med.unc.edu
|
Organization name |
University of North Carolina
|
Department |
Department of Genetics
|
Street address |
UNC Lineberger Cancer Center, 450 West Drive, CB #7295
|
City |
Chapel Hill |
State/province |
NC |
ZIP/Postal code |
27599-7295 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (2) |
GSE32283 |
Mutations in IDH1 are associated with DNA hypermethylation in glioblastomas |
GSE32286 |
DNA hypermethylation in intrahepatic cholangiocarcinomas and glioblastomas |
|