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Sample GSM798474 Query DataSets for GSM798474
Status Public on Apr 29, 2012
Title Treg WT rep2
Sample type RNA
Source name wild type Treg, replicate 2
Organism Mus musculus
Characteristics strain: C57BL/6
gender: Female
age: 6 weeks old
cell type: Treg
treatment: unstimulated
genotype: WT
Treatment protocol Stimulations were done with plate-coated anti-CD3(1mg/ml) and anti-CD28 (1mg/ml)for 13 hours.
Growth protocol Mice were maintained under specific pathogen-free conditions according to institutional guidelines under approved protocols in Kyoto University.
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted with Qiagen RNeasy mini kit with on-column DNaseI treatment. RNA quality was assessed using Agilent 2100 Bioanalyzer according to manufacturer's instructions.
Label Cy3
Label protocol Cy3 labeled cRNA was produced using Agilent Low Input QuickAmp Labeling Kit according to manufacturer's protocol.
Hybridization protocol For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
Scan protocol Slide were washed and then scanned with an Agilent G2505B scanner
Data processing Data were obtained and processed with the Agilent Feature Extraction Software(v9.5). The resulting data were imported into Expressionist and quantile normalized intensities were exported.
Submission date Sep 19, 2011
Last update date Apr 29, 2012
Contact name Tasuku Honjo
Organization name Kyoto University
Department Department of Immunology and Genomic Medicine
Street address Yoshida Konoe-cho, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8501
Country Japan
Platform ID GPL7202
Series (1)
GSE32224 Comparison of gene expression in Wild type and T cell-specific conditional Trim28 KO in TCR stimulated and un-stimulated naive CD4 positive and T regulatory cells

Data table header descriptions
VALUE Quantile normalized intensities of samples exported from Expressionist

Data table
A_51_P100021 97.10453
A_51_P100034 3889.604
A_51_P100052 3.541342
A_51_P100063 2.395408
A_51_P100084 11.06581
A_51_P100099 48.26474
A_51_P100155 743.1862
A_51_P100174 250.7546
A_51_P100181 13.51295
A_51_P100218 1.280315
A_51_P100227 190.0985
A_51_P100238 1.492577
A_51_P100246 167.6527
A_51_P100289 1457.16
A_51_P100298 1.695439
A_51_P100309 1.548735
A_51_P100327 223.8709
A_51_P100347 37.14016
A_51_P100379 7.703142
A_51_P100428 1.66764

Total number of rows: 41174

Table truncated, full table size 878 Kbytes.

Supplementary file Size Download File type/resource
GSM798474_Treg_wildtype_2.txt.gz 7.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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