|
Status |
Public on Apr 29, 2012 |
Title |
Treg WT rep1 |
Sample type |
RNA |
|
|
Source name |
wild type Treg, replicate 1
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 gender: Female age: 6 weeks old cell type: Treg treatment: unstimulated genotype: WT
|
Treatment protocol |
Stimulations were done with plate-coated anti-CD3(1mg/ml) and anti-CD28 (1mg/ml)for 13 hours.
|
Growth protocol |
Mice were maintained under specific pathogen-free conditions according to institutional guidelines under approved protocols in Kyoto University.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNAs were extracted with Qiagen RNeasy mini kit with on-column DNaseI treatment. RNA quality was assessed using Agilent 2100 Bioanalyzer according to manufacturer's instructions.
|
Label |
Cy3
|
Label protocol |
Cy3 labeled cRNA was produced using Agilent Low Input QuickAmp Labeling Kit according to manufacturer's protocol.
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|
|
Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
Scan protocol |
Slide were washed and then scanned with an Agilent G2505B scanner
|
Data processing |
Data were obtained and processed with the Agilent Feature Extraction Software(v9.5). The resulting data were imported into Expressionist and quantile normalized intensities were exported.
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|
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Submission date |
Sep 19, 2011 |
Last update date |
Apr 29, 2012 |
Contact name |
Tasuku Honjo |
E-mail(s) |
honjo@mfour.med.kyoto-u.ac.jp
|
Organization name |
Kyoto University
|
Department |
Department of Immunology and Genomic Medicine
|
Street address |
Yoshida Konoe-cho, Sakyo-ku
|
City |
Kyoto |
ZIP/Postal code |
606-8501 |
Country |
Japan |
|
|
Platform ID |
GPL7202 |
Series (1) |
GSE32224 |
Comparison of gene expression in Wild type and T cell-specific conditional Trim28 KO in TCR stimulated and un-stimulated naive CD4 positive and T regulatory cells |
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