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Sample GSM798467 Query DataSets for GSM798467
Status Public on Apr 29, 2012
Title Trim28CKO unstimulated CD4 positve T cell rep3
Sample type RNA
Source name Trim28 conditional KO mice naïve CD4 positive cell, unstimulated, replicate 3
Organism Mus musculus
Characteristics strain: C57BL/6
gender: Female
age: 6 weeks old
cell type: CD4 positive T cell
treatment: unstimulated
genotype: Trim28CKO
Treatment protocol Stimulations were done with plate-coated anti-CD3(1mg/ml) and anti-CD28 (1mg/ml)for 13 hours.
Growth protocol Mice were maintained under specific pathogen-free conditions according to institutional guidelines under approved protocols in Kyoto University.
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted with Qiagen RNeasy mini kit with on-column DNaseI treatment. RNA quality was assessed using Agilent 2100 Bioanalyzer according to manufacturer's instructions.
Label Cy3
Label protocol Cy3 labeled cRNA was produced using Agilent Low Input QuickAmp Labeling Kit according to manufacturer's protocol.
Hybridization protocol For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
Scan protocol Slide were washed and then scanned with an Agilent G2505B scanner
Data processing Data were obtained and processed with the Agilent Feature Extraction Software(v9.5). The resulting data were imported into Expressionist and quantile normalized intensities were exported.
Submission date Sep 19, 2011
Last update date Apr 29, 2012
Contact name Tasuku Honjo
Organization name Kyoto University
Department Department of Immunology and Genomic Medicine
Street address Yoshida Konoe-cho, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8501
Country Japan
Platform ID GPL7202
Series (1)
GSE32224 Comparison of gene expression in Wild type and T cell-specific conditional Trim28 KO in TCR stimulated and un-stimulated naive CD4 positive and T regulatory cells

Data table header descriptions
VALUE Quantile normalized intensities of samples exported from Expressionist

Data table
A_51_P100021 31.14837
A_51_P100034 2138.196
A_51_P100052 3.056678
A_51_P100063 6.48762
A_51_P100084 5.40114
A_51_P100099 69.38441
A_51_P100155 911.8608
A_51_P100174 325.1124
A_51_P100181 16.28923
A_51_P100218 1.257751
A_51_P100227 162.0823
A_51_P100238 1.353609
A_51_P100246 132.8959
A_51_P100289 1528.208
A_51_P100298 1.501959
A_51_P100309 1.716117
A_51_P100327 220.0592
A_51_P100347 38.69158
A_51_P100379 9.73829
A_51_P100428 1.496754

Total number of rows: 41174

Table truncated, full table size 878 Kbytes.

Supplementary file Size Download File type/resource
GSM798467_KO_unstimulated_3.txt.gz 7.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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