GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM797648 Query DataSets for GSM797648
Status Public on Nov 30, 2011
Title MIWI -/KI biological replicate 1
Sample type RNA
Source name purified Round Spermatids total RNA, adult
Organism Mus musculus
Characteristics genotype/variation: MIWI -/KI
strain: C57BL/6
Treatment protocol removal tunica albuginea, collagenase treatment in PBS, cell extraction from seminiferous tubules by pipetting with pasteur pipette, cell fractionation with BSA gradient 2-4%
Growth protocol no specific treatment of mice, testes dissection at room temperature, testes kept in cold PBS before cell fractionation
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA from round spermatids pooled from the respective gradient fractions
Label biotin
Label protocol Affymetrix protocol
Hybridization protocol Affymetrix protocol
Scan protocol Affymetrix protocol
Data processing The data were analyzed with RMA from aroma.affy normalization method.
Submission date Sep 16, 2011
Last update date Nov 30, 2011
Contact name Ramesh Pillai
Organization name University of Geneva
Department Department of Molecular Biology
Street address 30, Quai Ernest-Ansermet
City Gneveva
ZIP/Postal code CH-1211
Country Switzerland
Platform ID GPL6246
Series (2)
GSE32180 MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [microarray]
GSE32183 MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing

Data table header descriptions
VALUE RMA signal intensities

Data table
10338001 1776.91455078123
10338003 575.770812988281
10338004 220.254653930664
10338017 3001.5620117187
10338025 166.659286499023
10338026 3438.33129882817
10338029 336.463439941406
10338035 236.441802978516
10338036 220.500625610351
10338037 15.7739591598511
10338041 638.505615234375
10338042 363.837158203124
10338044 1419.11328125
10338047 30.1284065246582
10338056 13.0148620605469
10338059 5028.86865234365
10338060 14.5962314605713
10338063 1
10338064 20.5321178436279
10338065 24.6675930023193

Total number of rows: 35512

Table truncated, full table size 897 Kbytes.

Supplementary file Size Download File type/resource
GSM797648.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap