|
| Status |
Public on Sep 01, 2013 |
| Title |
del175k mutant_ChIPSeq |
| Sample type |
SRA |
| |
|
| Source name |
osteoblast
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: del175k mutant
antibody: sc-30486
vendor: Santa Cruz biotechnology
strain: 129sv
cell type: primary osteoblast
|
| Growth protocol |
The primary osteoblasts of Tnni2del175k/175k mice and wild-type littermates was used for ChiP-Seq
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
The part of ChIP assays in ChIP-seq study are performed as the protocol of this paper, Schmidt, D. et al., ChIP-seq: using high-throughput sequencing to discover protein-DNA interactions. Methods 48 (3), 240-248 (2009).
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Chromatin IP against tnni2
|
| Data processing |
Raw reads were aligned with the mouse reference 8 using Bowtie software and viewed with the IGV browser.
|
| |
|
| Submission date |
Sep 15, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
xiaoquan zhu |
| E-mail(s) |
zxq_gy@yahoo.com.cn
|
| Organization name |
beijing hospital
|
| Street address |
N01 dahua road dongdan beijing
|
| City |
beijing |
| ZIP/Postal code |
100730 |
| Country |
China |
| |
|
| Platform ID |
GPL13112 |
| Series (2) |
| GSE32166 |
tnni2 Binding Genome Seqences |
| GSE32401 |
Tnni2del175k mutant mice and their wild-type littermates using mRNA array and ChIP-seq |
|
| Relations |
| SRA |
SRX099194 |
| BioSample |
SAMN00727929 |
