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Status |
Public on Sep 01, 2013 |
Title |
bone_wild-type_rep2 |
Sample type |
RNA |
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|
Source name |
bone of newbone mice
|
Organism |
Mus musculus |
Characteristics |
tissue: bone genotype/variation: wild-type strain: 129sv
|
Growth protocol |
3 paires of newbone mice radii and ulnae from Tnni2del175k mutant mice and their wild-type littermates were collected
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated with the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.recoveries of long transcripts.
|
Label |
biotin
|
Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
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|
|
Hybridization protocol |
Samples were hybridized with GeneChip Mouse Gene 1.0 ST (Affymetrix) and scanned at the UCLA Microarray Core Facility.
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Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
Data processing |
The raw data were processed using SUM software 3.02.
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Submission date |
Sep 15, 2011 |
Last update date |
Sep 01, 2013 |
Contact name |
xiaoquan zhu |
E-mail(s) |
zxq_gy@yahoo.com.cn
|
Organization name |
beijing hospital
|
Street address |
N01 dahua road dongdan beijing
|
City |
beijing |
ZIP/Postal code |
100730 |
Country |
China |
|
|
Platform ID |
GPL6246 |
Series (2) |
GSE32163 |
The study of the differential expression profile in bone tissues between Tnni2del175k mutant mice and their wild-type littermates using mRNA array. |
GSE32401 |
Tnni2del175k mutant mice and their wild-type littermates using mRNA array and ChIP-seq |
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