 |
 |
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Feb 15, 2024 |
Title |
ILC, D14, scRNAseq |
Sample type |
SRA |
|
|
Source name |
Lung
|
Organism |
Mus musculus |
Characteristics |
tissue: Lung cell type: ILCs strain: C57BL/6 treatment: Mtb infection d14
|
Extracted molecule |
total RNA |
Extraction protocol |
Lung single cell suspension was prepared by digesting the heart. After lysing red blood cells, single cells were subjected to staining with antobodies to to ILCs. Sorted ILCs were then resuspended in 0.04% w/v BSA in PBS and sunjected to 10X pipeline. 10x 3' GEX (v2)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic single cell |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
10X genomics
|
Data processing |
demultiplexing, alignment, raw gene expression matrices were by the Cell Ranger software (10x Genomics, version 3) available on the 10x website Assembly: mm10 Supplementary files format and content: tab seperated values files and matrix files
|
|
|
Submission date |
Dec 08, 2023 |
Last update date |
Feb 15, 2024 |
Contact name |
Shabaana Khader |
E-mail(s) |
khader@uchicago.edu
|
Organization name |
The University of Chicago
|
Street address |
920 East 58th Street
|
City |
Chicago |
State/province |
Illinois |
ZIP/Postal code |
60637 |
Country |
USA |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE249667 |
Characterisation of ILCs isolated from Mtb infected mice lungs |
|
Relations |
BioSample |
SAMN38726257 |
SRA |
SRX22825994 |
Supplementary file |
Size |
Download |
File type/resource |
GSM7957027_D14_barcodes.tsv.gz |
21.8 Kb |
(ftp)(http) |
TSV |
GSM7957027_D14_features.tsv.gz |
284.1 Kb |
(ftp)(http) |
TSV |
GSM7957027_D14_matrix.mtx.gz |
20.8 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
Raw data are available in SRA |
|
|
|
|
 |