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Sample GSM7957026 Query DataSets for GSM7957026
Status Public on Feb 15, 2024
Title ILC, D5, scRNAseq
Sample type SRA
 
Source name Lung
Organism Mus musculus
Characteristics tissue: Lung
cell type: ILCs
strain: C57BL/6
treatment: Mtb infection d5
Extracted molecule total RNA
Extraction protocol Lung single cell suspension was prepared by digesting the heart. After lysing red blood cells, single cells were subjected to staining with antobodies to to ILCs. Sorted ILCs were then resuspended in 0.04% w/v BSA in PBS and sunjected to 10X pipeline.
10x 3' GEX (v2)
 
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description 10X genomics
Data processing demultiplexing, alignment, raw gene expression matrices were by the Cell Ranger software (10x Genomics, version 3) available on the 10x website
Assembly: mm10
Supplementary files format and content: tab seperated values files and matrix files
 
Submission date Dec 08, 2023
Last update date Feb 15, 2024
Contact name Shabaana Khader
E-mail(s) khader@uchicago.edu
Organization name The University of Chicago
Street address 920 East 58th Street
City Chicago
State/province Illinois
ZIP/Postal code 60637
Country USA
 
Platform ID GPL24247
Series (1)
GSE249667 Characterisation of ILCs isolated from Mtb infected mice lungs
Relations
BioSample SAMN38726258
SRA SRX22825993

Supplementary file Size Download File type/resource
GSM7957026_D5_barcodes.tsv.gz 14.6 Kb (ftp)(http) TSV
GSM7957026_D5_features.tsv.gz 284.1 Kb (ftp)(http) TSV
GSM7957026_D5_matrix.mtx.gz 12.0 Mb (ftp)(http) MTX
SRA Run SelectorHelp
Raw data are available in SRA

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