|
| Status |
Public on Dec 01, 2011 |
| Title |
GIST-T1 cells with siHOTAIR |
| Sample type |
RNA |
| |
|
| Source name |
siRNA targeting HOTAIR
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: GIST-T1
cell type: gastrointestinal stromal tumor
sirna: siRNA targeting HOTAIR
|
| Treatment protocol |
GIST-T1 cells in 6-well plates were transfected with 100 pmol of Stealth siRNA (Invitrogen) or a Stealth RNAi Negative Control Medium GC (Invitrogen) using Lipofectamine2000 (Invitrogen).
|
| Growth protocol |
GIST-T1 cells were maintained in DMEM supplemented with 10% fatal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol Reagent (Invitrogen).
|
| Label |
Cy3
|
| Label protocol |
Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
|
| |
|
| Hybridization protocol |
Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
|
| Scan protocol |
Array was scanned with an Agilent G2565BA Microarray Scanner.
|
| Description |
gene expression in GIST-T1 cells transfected with siRNA targeting HOTAIR
|
| Data processing |
Agilent Feature Extraction Software (v 10.7) was used for background subtraction.
|
| |
|
| Submission date |
Sep 12, 2011 |
| Last update date |
Dec 01, 2011 |
| Contact name |
Hiromu Suzuki |
| E-mail(s) |
hsuzuki@sapmed.ac.jp
|
| Phone |
+81-11-611-2111
|
| Organization name |
Sapporo Medical University
|
| Department |
Department of Molecular Biology
|
| Street address |
S1, W17, Chuo-ku
|
| City |
Sapporo |
| State/province |
Hokkaido |
| ZIP/Postal code |
060-8556 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE32064 |
Gene expression signatures in GIST-T1 cells transfected with siRNA targeting HOTAIR |
|
