|
Status |
Public on Dec 01, 2011 |
Title |
GIST-T1 cells with siCONT |
Sample type |
RNA |
|
|
Source name |
control siRNA
|
Organism |
Homo sapiens |
Characteristics |
cell line: GIST-T1 cell type: gastrointestinal stromal tumor sirna: control siRNA
|
Treatment protocol |
GIST-T1 cells in 6-well plates were transfected with 100 pmol of Stealth siRNA (Invitrogen) or a Stealth RNAi Negative Control Medium GC (Invitrogen) using Lipofectamine2000 (Invitrogen).
|
Growth protocol |
GIST-T1 cells were maintained in DMEM supplemented with 10% fatal bovine serum.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Trizol Reagent (Invitrogen).
|
Label |
Cy3
|
Label protocol |
Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
|
|
|
Hybridization protocol |
Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
|
Scan protocol |
Array was scanned with an Agilent G2565BA Microarray Scanner.
|
Description |
gene expression in GIST-T1 cells transfected with control siRNA
|
Data processing |
Agilent Feature Extraction Software (v 10.7) was used for background subtraction.
|
|
|
Submission date |
Sep 12, 2011 |
Last update date |
Dec 01, 2011 |
Contact name |
Hiromu Suzuki |
E-mail(s) |
hsuzuki@sapmed.ac.jp
|
Phone |
+81-11-611-2111
|
Organization name |
Sapporo Medical University
|
Department |
Department of Molecular Biology
|
Street address |
S1, W17, Chuo-ku
|
City |
Sapporo |
State/province |
Hokkaido |
ZIP/Postal code |
060-8556 |
Country |
Japan |
|
|
Platform ID |
GPL13607 |
Series (1) |
GSE32064 |
Gene expression signatures in GIST-T1 cells transfected with siRNA targeting HOTAIR |
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