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Sample GSM795165 Query DataSets for GSM795165
Status Public on Dec 01, 2011
Title GIST-T1 cells with siCONT
Sample type RNA
Source name control siRNA
Organism Homo sapiens
Characteristics cell line: GIST-T1
cell type: gastrointestinal stromal tumor
sirna: control siRNA
Treatment protocol GIST-T1 cells in 6-well plates were transfected with 100 pmol of Stealth siRNA (Invitrogen) or a Stealth RNAi Negative Control Medium GC (Invitrogen) using Lipofectamine2000 (Invitrogen).
Growth protocol GIST-T1 cells were maintained in DMEM supplemented with 10% fatal bovine serum.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol Reagent (Invitrogen).
Label Cy3
Label protocol Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
Hybridization protocol Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
Scan protocol Array was scanned with an Agilent G2565BA Microarray Scanner.
Description gene expression in GIST-T1 cells transfected with control siRNA
Data processing Agilent Feature Extraction Software (v 10.7) was used for background subtraction.
Submission date Sep 12, 2011
Last update date Dec 01, 2011
Contact name Hiromu Suzuki
Phone +81-11-611-2111
Organization name Sapporo Medical University
Department Department of Molecular Biology
Street address S1, W17, Chuo-ku
City Sapporo
State/province Hokkaido
ZIP/Postal code 060-8556
Country Japan
Platform ID GPL13607
Series (1)
GSE32064 Gene expression signatures in GIST-T1 cells transfected with siRNA targeting HOTAIR

Data table header descriptions
VALUE processed Cy3 signal intensity (Agilent gProcessedSignal)

Data table
1 4.227965e+004
2 1.055045e+001
3 1.061306e+001
4 2.947163e+002
5 1.086510e+003
6 5.214484e+001
7 1.437072e+004
8 1.193843e+003
9 1.088911e+001
10 1.194276e+001
11 1.094518e+001
12 6.757170e+002
13 6.382762e+003
14 5.657960e+002
15 1.104113e+004
16 1.101952e+001
17 6.524153e+001
18 1.102433e+001
19 1.102304e+001
20 1.333894e+003

Total number of rows: 62976

Table truncated, full table size 1219 Kbytes.

Supplementary file Size Download File type/resource
GSM795165_GIST-T1_siCONT.txt.gz 12.2 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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