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Sample GSM7949483 Query DataSets for GSM7949483
Status Public on Jan 24, 2024
Title 3226, Control, bio rep 2
Sample type SRA
 
Source name 3226
Organism Homo sapiens
Characteristics cell line: 3226
treatment: DMSO
Treatment protocol The cells were treated with 0.1% DMSO and MRTX1133 (500 nM) up to 48 hours
Growth protocol Cells were grown in Keratinocyte Serum-Free media (KSF) supplemented with 2% FBS, EGF (0.2 ng/mL) and bovine pituitary extract (30 µg/mL) (Life Technologies, Carlsbad, CA)
Extracted molecule total RNA
Extraction protocol RNA was extracted using Quiagen Rneasy plus mini Kit.
After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description A8
Data processing nf-core/rnaseq version 3.3.
Assembly: GRCh38
Supplementary files format and content: Tab delimited text files, contain raw read counts at isoform level.
 
Submission date Dec 06, 2023
Last update date Jan 24, 2024
Contact name Erik Knudsen
E-mail(s) Erik.Knudsen@RoswellPark.org
Organization name Roswell Park Comprehensive Cancer Center
Department Molecular and Cellular Biology
Street address 665 Elm Street
City Buffalo
State/province NY
ZIP/Postal code 14203
Country USA
 
Platform ID GPL11154
Series (2)
GSE249539 Extracellular niche and tumor microenvironment enhance the impact of KRAS inhibitors in pancreatic cancer models [RNA-seq]
GSE249541 Extracellular niche and tumor microenvironment enhance the impact of KRAS inhibitors in pancreatic cancer models.
Relations
BioSample SAMN38699628
SRA SRX22806517

Supplementary file Size Download File type/resource
GSM7949483_3226_DMSO_8.isoforms.results.gz 1.2 Mb (ftp)(http) RESULTS
SRA Run SelectorHelp
Raw data are available in SRA

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