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| Status |
Public on Jan 24, 2024 |
| Title |
1222, Control, bio rep 1 |
| Sample type |
SRA |
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| Source name |
1222
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| Organism |
Homo sapiens |
| Characteristics |
cell line: 1222
treatment: DMSO
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| Treatment protocol |
The cells were treated with 0.1% DMSO and MRTX1133 (500 nM) up to 48 hours
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| Growth protocol |
Cells were grown in Keratinocyte Serum-Free media (KSF) supplemented with 2% FBS, EGF (0.2 ng/mL) and bovine pituitary extract (30 µg/mL) (Life Technologies, Carlsbad, CA)
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Quiagen Rneasy plus mini Kit.
After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Description |
A1
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| Data processing |
nf-core/rnaseq version 3.3.
Assembly: GRCh38
Supplementary files format and content: Tab delimited text files, contain raw read counts at isoform level.
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| Submission date |
Dec 06, 2023 |
| Last update date |
Jan 24, 2024 |
| Contact name |
Jianxin Wang |
| Organization name |
Roswell Park Comprehensive Cancer Center
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| Department |
Molecular and Cellular Biology
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| Street address |
665 Elm Street
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| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14203 |
| Country |
USA |
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| Platform ID |
GPL11154 |
| Series (2) |
| GSE249539 |
Extracellular niche and tumor microenvironment enhance the impact of KRAS inhibitors in pancreatic cancer models [RNA-seq] |
| GSE249541 |
Extracellular niche and tumor microenvironment enhance the impact of KRAS inhibitors in pancreatic cancer models. |
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| Relations |
| BioSample |
SAMN38699635 |
| SRA |
SRX22806510 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM7949476_1222_DMSO_1.isoforms.results.gz |
1.2 Mb |
(ftp)(http) |
RESULTS |
SRA Run Selector |
| Raw data are available in SRA |
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